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1mug

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G:T/U MISMATCH-SPECIFIC DNA GLYCOSYLASE FROM E.COLI

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Retrieved from "http://52.214.119.220/wiki/index.php/1mug"

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-[[Image:1mug.gif|left|200px]]<br /><applet load="1mug" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1mug, resolution 1.80&Aring;" />
-'''G:T/U MISMATCH-SPECIFIC DNA GLYCOSYLASE FROM E.COLI'''<br />
-==Overview==
+==G:T/U MISMATCH-SPECIFIC DNA GLYCOSYLASE FROM E.COLI==
-G:U mismatches resulting from deamination of cytosine are the most common, promutagenic lesions occurring in DNA. Uracil is removed in a, base-excision repair pathway by uracil DNA-glycosylase (UDG), which, excises uracil from both single- and double-stranded DNA. Recently, a, biochemically distinct family of DNA repair enzymes has been identified, which excises both uracil and thymine, but only from mispairs with, guanine. Crystal structures of the mismatch-specific uracil, DNA-glycosylase (MUG) from E. coli, and of a DNA complex, reveal a, remarkable structural and functional homology to UDGs despite low sequence, identity. Details of the MUG structure explain its thymine DNA-glycosylase, activity and the specificity for G:U/T mispairs, which derives from direct, recognition of guanine on the complementary strand.
+<StructureSection load='1mug' size='340' side='right'caption='[[1mug]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1mug]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MUG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MUG FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mug OCA], [https://pdbe.org/1mug PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mug RCSB], [https://www.ebi.ac.uk/pdbsum/1mug PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mug ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/MUG_ECOLI MUG_ECOLI] Excises ethenocytosine and uracil, which can arise by alkylation or deamination of cytosine, respectively, from the corresponding mispairs with guanine in ds-DNA. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base. The complementary strand guanine functions in substrate recognition. Required for DNA damage lesion repair in stationary-phase cells.<ref>PMID:8878487</ref> <ref>PMID:12668677</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mu/1mug_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mug ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-MUG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MUG OCA].
+*[[DNA glycosylase 3D structures|DNA glycosylase 3D structures]]
+== References ==
-==Reference==
+<references/>
-Crystal structure of a G:T/U mismatch-specific DNA glycosylase: mismatch recognition by complementary-strand interactions., Barrett TE, Savva R, Panayotou G, Barlow T, Brown T, Jiricny J, Pearl LH, Cell. 1998 Jan 9;92(1):117-29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9489705 9489705]
+__TOC__
+</StructureSection>
 [[Category: Escherichia coli]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Barlow, T.]]
+[[Category: Barlow T]]
-[[Category: Barrett, T.E.]]
+[[Category: Barrett TE]]
-[[Category: Brown, T.]]
+[[Category: Brown T]]
-[[Category: Jiricny, J.]]
+[[Category: Jiricny J]]
-[[Category: Panayotou, G.]]
+[[Category: Panayotou G]]
-[[Category: Pearl, L.H.]]
+[[Category: Pearl LH]]
-[[Category: Savva, R.]]
+[[Category: Savva R]]
-[[Category: SO4]]
-[[Category: dna-glycosylase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:42:33 2007''

1mug

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G:T/U MISMATCH-SPECIFIC DNA GLYCOSYLASE FROM E.COLI

Structural highlights

Function

Evolutionary Conservation

See Also

References

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