1mwe

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(New page: 200px<br /><applet load="1mwe" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mwe, resolution 1.7&Aring;" /> '''THE X-RAY STRUCTURE O...)
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[[Image:1mwe.gif|left|200px]]<br /><applet load="1mwe" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mwe, resolution 1.7&Aring;" />
 
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'''THE X-RAY STRUCTURE OF A COMPLEX OF TERN N9 INFLUENZA VIRUS NEURAMINIDASE COMPLEXED WITH SIALIC ACID AT 4 DEGREES C REVEALING A SECOND SIALIC ACID BINDING SITE'''<br />
 
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==Overview==
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==THE X-RAY STRUCTURE OF A COMPLEX OF TERN N9 INFLUENZA VIRUS NEURAMINIDASE COMPLEXED WITH SIALIC ACID AT 4 DEGREES C REVEALING A SECOND SIALIC ACID BINDING SITE==
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The x-ray structure of a complex of sialic acid (Neu5Ac) with, neuraminidase N9 subtype from A/tern/Australia/G70C/75 influenza virus at, 4 degrees C has revealed the location of a second Neu5Ac binding site on, the surface of the enzyme. At 18 degrees C, only the enzyme active site, contains bound Neu5Ac. Neu5Ac binds in the second site in the chair, conformation in a similar way to which it binds to hemagglutinin. The, residues that interact with Neu5Ac at this second site are mostly, conserved in avian strains, but not in human and swine strains, indicating, that it has some as-yet-unknown biological function in birds.
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<StructureSection load='1mwe' size='340' side='right'caption='[[1mwe]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mwe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_A_virus Influenza A virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MWE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mwe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mwe OCA], [https://pdbe.org/1mwe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mwe RCSB], [https://www.ebi.ac.uk/pdbsum/1mwe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mwe ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NRAM_I75A5 NRAM_I75A5] Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Otherwise, infection would be limited to one round of replication. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. May facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells. Likely to plays a role in the budding process through its association with lipid rafts during intracellular transport. May additionally display a raft-association independent effect on budding. Plays a role in the determination of host range restriction on replication and virulence. Sialidase activity in late endosome/lysosome traffic seems to enhance virus replication.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mw/1mwe_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mwe ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1MWE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus] with NAG, SIA and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MWE OCA].
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*[[Neuraminidase 3D structures|Neuraminidase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural evidence for a second sialic acid binding site in avian influenza virus neuraminidases., Varghese JN, Colman PM, van Donkelaar A, Blick TJ, Sahasrabudhe A, McKimm-Breschkin JL, Proc Natl Acad Sci U S A. 1997 Oct 28;94(22):11808-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9342319 9342319]
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[[Category: Influenza A virus]]
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[[Category: Exo-alpha-sialidase]]
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[[Category: Large Structures]]
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[[Category: Influenza a virus]]
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[[Category: Varghese JN]]
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[[Category: Single protein]]
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[[Category: Varghese, J.N.]]
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[[Category: CA]]
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[[Category: NAG]]
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[[Category: SIA]]
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[[Category: avian influenza]]
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[[Category: hemagglutination site]]
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[[Category: heme absorbing site]]
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[[Category: hydrolase]]
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[[Category: neuraminidase]]
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[[Category: sialic acid binding]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:45:45 2007''
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Current revision

THE X-RAY STRUCTURE OF A COMPLEX OF TERN N9 INFLUENZA VIRUS NEURAMINIDASE COMPLEXED WITH SIALIC ACID AT 4 DEGREES C REVEALING A SECOND SIALIC ACID BINDING SITE

PDB ID 1mwe

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