1mwj

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1mwj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mwj, resolution 2.85&Aring;" /> '''Crystal Structure of...)
Current revision (07:49, 14 February 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1mwj.gif|left|200px]]<br /><applet load="1mwj" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1mwj, resolution 2.85&Aring;" />
 
-
'''Crystal Structure of a MUG-DNA pseudo substrate complex'''<br />
 
-
==Overview==
+
==Crystal Structure of a MUG-DNA pseudo substrate complex==
-
The bacterial mismatch-specific uracil-DNA glycosylase (MUG) and, eukaryotic thymine-DNA glycosylase (TDG) enzymes form a homologous family, of DNA glycosylases that initiate base-excision repair of G:U/T, mismatches. Despite low sequence homology, the MUG/TDG enzymes are, structurally related to the uracil-DNA glycosylase enzymes, but have a, very different mechanism for substrate recognition. We have now determined, the crystal structure of the Escherichia coli MUG enzyme complexed with an, oligonucleotide containing a non-hydrolysable deoxyuridine analogue, mismatched with guanine, providing the first structure of an intact, substrate-nucleotide productively bound to a hydrolytic DNA glycosylase., The structure of this complex explains the preference for G:U over G:T, mispairs, and reveals an essentially non-specific pyrimidine-binding, pocket that allows MUG/TDG enzymes to excise the alkylated base, 3, N(4)-ethenocytosine. Together with structures for the free enzyme and for, an abasic-DNA product complex, the MUG-substrate analogue complex reveals, the conformational changes accompanying the catalytic cycle of substrate, binding, base excision and product release.
+
<StructureSection load='1mwj' size='340' side='right'caption='[[1mwj]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1mwj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MWJ FirstGlance]. <br>
-
1MWJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MWJ OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
-
 
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mwj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mwj OCA], [https://pdbe.org/1mwj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mwj RCSB], [https://www.ebi.ac.uk/pdbsum/1mwj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mwj ProSAT]</span></td></tr>
-
==Reference==
+
</table>
-
Crystal structure of a thwarted mismatch glycosylase DNA repair complex., Barrett TE, Scharer OD, Savva R, Brown T, Jiricny J, Verdine GL, Pearl LH, EMBO J. 1999 Dec 1;18(23):6599-609. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10581234 10581234]
+
== Function ==
 +
[https://www.uniprot.org/uniprot/MUG_ECOLI MUG_ECOLI] Excises ethenocytosine and uracil, which can arise by alkylation or deamination of cytosine, respectively, from the corresponding mispairs with guanine in ds-DNA. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base. The complementary strand guanine functions in substrate recognition. Required for DNA damage lesion repair in stationary-phase cells.<ref>PMID:8878487</ref> <ref>PMID:12668677</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mw/1mwj_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mwj ConSurf].
 +
<div style="clear:both"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Barrett, T.E.]]
+
[[Category: Barrett TE]]
-
[[Category: Brown, T.]]
+
[[Category: Brown T]]
-
[[Category: Jiricny, J.]]
+
[[Category: Jiricny J]]
-
[[Category: Pearl, L.H.]]
+
[[Category: Pearl LH]]
-
[[Category: Savva, R.]]
+
[[Category: Savva R]]
-
[[Category: Scharer, O.]]
+
[[Category: Scharer O]]
-
[[Category: Verdine, G.L.]]
+
[[Category: Verdine GL]]
-
[[Category: non-hydrolysable dna-complex]]
+
-
[[Category: rossmann fold]]
+
-
[[Category: uracil recognition.]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:45:56 2007''
+

Current revision

Crystal Structure of a MUG-DNA pseudo substrate complex

PDB ID 1mwj

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1mwj"
Personal tools