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1mzl

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(New page: 200px<br /><applet load="1mzl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mzl, resolution 1.9&Aring;" /> '''MAIZE NONSPECIFIC LIP...)
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[[Image:1mzl.jpg|left|200px]]<br /><applet load="1mzl" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mzl, resolution 1.9&Aring;" />
 
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'''MAIZE NONSPECIFIC LIPID TRANSFER PROTEIN'''<br />
 
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==Overview==
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==MAIZE NONSPECIFIC LIPID TRANSFER PROTEIN==
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BACKGROUND: The movement of lipids between membranes is aided by, lipid-transfer proteins (LTPs). Some LTPs exhibit broad specificity, transferring many classes of lipids, and are termed non-specific LTPs, (ns-LTPs). Despite their apparently similar mode of action, no sequence, homology exists between mammalian and plant ns-LTPs and no, three-dimensional structure has been reported for any plant ns-LTP., RESULTS: We have determined the crystal structure of ns-LTP from maize, seedlings by multiple isomorphous replacement and refined the structure to, 1.9 A resolution. The protein comprises a single compact domain with four, alpha-helices and a long C-terminal region. The eight conserved cysteines, form four disulfide bridges (assigned as Cys4-Cys52, Cys14-Cys29, Cys30-Cys75, and Cys50-Cys89) resolving the ambiguity that remained from, the chemical determination of pairings in the homologous protein from, castor bean. Two of the bonds, Cys4-Cys52 and Cys50-Cys89, differ from, what would have been predicted from sequence alignment with soybean, hydrophobic protein. The complex between maize ns-LTP and hexadecanoate, (palmitate) has also been crystallized and its structure refined to 1.8 A, resolution. CONCLUSIONS: The fold of maize ns-LTP places it in a new, category of all-alpha-type structure, first described for soybean, hydrophobic protein. In the absence of a bound ligand, the protein has a, tunnel-like hydrophobic cavity, which is large enough to accommodate a, long fatty acyl chain. In the structure of the complex with palmitate, most of the acyl chain is buried inside this hydrophobic cavity.
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<StructureSection load='1mzl' size='340' side='right'caption='[[1mzl]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1mzl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MZL FirstGlance]. <br>
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1MZL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MZL OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mzl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mzl OCA], [https://pdbe.org/1mzl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mzl RCSB], [https://www.ebi.ac.uk/pdbsum/1mzl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mzl ProSAT]</span></td></tr>
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==Reference==
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</table>
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High-resolution crystal structure of the non-specific lipid-transfer protein from maize seedlings., Shin DH, Lee JY, Hwang KY, Kim KK, Suh SW, Structure. 1995 Feb 15;3(2):189-99. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7735835 7735835]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/NLTP_MAIZE NLTP_MAIZE] Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mz/1mzl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mzl ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Zea mays]]
[[Category: Zea mays]]
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[[Category: Hwang, K.Y.]]
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[[Category: Hwang KY]]
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[[Category: Kim, K.K.]]
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[[Category: Kim KK]]
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[[Category: Lee, J.Y.]]
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[[Category: Lee JY]]
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[[Category: Shin, D.H.]]
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[[Category: Shin DH]]
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[[Category: Suh, S.W.]]
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[[Category: Suh SW]]
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[[Category: alpha-helical structure]]
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[[Category: lipid transport]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:50:00 2007''
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Current revision

MAIZE NONSPECIFIC LIPID TRANSFER PROTEIN

PDB ID 1mzl

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