1n03

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(New page: 200px<br /><applet load="1n03" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n03" /> '''Model for Active RecA Filament'''<br /> ==O...)
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[[Image:1n03.gif|left|200px]]<br /><applet load="1n03" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1n03" />
 
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'''Model for Active RecA Filament'''<br />
 
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==Overview==
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==Model for Active RecA Filament==
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The crystal structure of the E. coli RecA protein was solved more than 10, years ago, but it has provided limited insight into the mechanism of, homologous genetic recombination. Using electron microscopy, we have, reconstructed five different states of RecA-DNA filaments. The C-terminal, lobe of the RecA protein is modulated by the state of the distantly bound, nucleotide, and this allosteric coupling can explain how mutations and, truncations of this C-terminal lobe enhance RecA's activity. A model, generated from these reconstructions shows that the nucleotide binding, core is substantially rotated from its position in the RecA crystal, filament, resulting in ATP binding between subunits. This simple rotation, can explain the large cooperativity in ATP hydrolysis observed for, RecA-DNA filaments.
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<SX load='1n03' size='340' side='right' viewer='molstar' caption='[[1n03]], [[Resolution|resolution]] 20.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1n03]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N03 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N03 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 20&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n03 OCA], [https://pdbe.org/1n03 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n03 RCSB], [https://www.ebi.ac.uk/pdbsum/1n03 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n03 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RECA_ECOLI RECA_ECOLI] Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage.[HAMAP-Rule:MF_00268]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n0/1n03_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n03 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1N03 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ADP as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1N03 OCA].
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*[[3D structures of recombinase A|3D structures of recombinase A]]
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__TOC__
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==Reference==
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</SX>
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ATP-mediated conformational changes in the RecA filament., VanLoock MS, Yu X, Yang S, Lai AL, Low C, Campbell MJ, Egelman EH, Structure. 2003 Feb;11(2):187-96. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12575938 12575938]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Campbell, M.J.]]
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[[Category: Campbell MJ]]
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[[Category: Egelman, E.H.]]
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[[Category: Egelman EH]]
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[[Category: Lai, A.L.]]
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[[Category: Lai AL]]
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[[Category: Low, C.]]
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[[Category: Low C]]
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[[Category: VanLoock, M.S.]]
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[[Category: VanLoock MS]]
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[[Category: Yang, S.]]
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[[Category: Yang S]]
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[[Category: Yu, X.]]
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[[Category: Yu X]]
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[[Category: ADP]]
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[[Category: helical polymer]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:50:40 2007''
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Current revision

Model for Active RecA Filament

1n03, resolution 20.00Å

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