1n0i

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(New page: 200px<br /><applet load="1n0i" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n0i, resolution 2.00&Aring;" /> '''Crystal Structure of...)
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[[Image:1n0i.gif|left|200px]]<br /><applet load="1n0i" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1n0i, resolution 2.00&Aring;" />
 
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'''Crystal Structure of Ferrochelatase with Cadmium bound at active site'''<br />
 
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==Overview==
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==Crystal Structure of Ferrochelatase with Cadmium bound at active site==
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Ferrochelatase, the terminal enzyme in heme biosynthesis, catalyses metal, insertion into protoporphyrin IX. The location of the metal binding site, with respect to the bound porphyrin substrate and the mode of metal, binding are of central importance for understanding the mechanism of, porphyrin metallation. In this work we demonstrate that Zn(2+), which is, commonly used as substrate in assays of the ferrochelatase reaction, and, Cd(2+), an inhibitor of the enzyme, bind to the invariant amino acids, His183 and Glu264 and water molecules, all located within the porphyrin, binding cleft. On the other hand, Mg(2+), which has been shown to bind, close to the surface at 7 A from His183, was largely absent from its site., Activity measurements demonstrate that Mg(2+) has a stimulatory effect on, the enzyme, lowering K(M) for Zn(2+) from 55 to 24 micro M. Changing one, of the Mg(2+) binding residues, Glu272, to serine abolishes the effect of, Mg(2+). It is proposed that prior to metal insertion the metal may form a, sitting-atop (SAT) complex with the invariant His-Glu couple and the, porphyrin. Metal binding to the Mg(2+) site may stimulate metal release, from the protein ligands and its insertion into the porphyrin.
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<StructureSection load='1n0i' size='340' side='right'caption='[[1n0i]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1n0i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N0I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N0I FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n0i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n0i OCA], [https://pdbe.org/1n0i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n0i RCSB], [https://www.ebi.ac.uk/pdbsum/1n0i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n0i ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CPFC_BACSU CPFC_BACSU] Involved in coproporphyrin-dependent heme b biosynthesis (PubMed:25646457, PubMed:25908396). Catalyzes the insertion of ferrous iron into coproporphyrin III to form Fe-coproporphyrin III (PubMed:25646457, PubMed:25908396). It can also insert iron into protoporphyrin IX (PubMed:1459957, PubMed:8119288, PubMed:21052751, PubMed:25646457). Has weaker activity with 2,4 disulfonate, deuteroporphyrin and 2,4 hydroxyethyl (PubMed:25646457, PubMed:12761666). In vitro, can also use Zn(2+) or Cu(2+) (PubMed:8119288, PubMed:16140324, PubMed:21052751, PubMed:12761666).<ref>PMID:12761666</ref> <ref>PMID:1459957</ref> <ref>PMID:16140324</ref> <ref>PMID:21052751</ref> <ref>PMID:25646457</ref> <ref>PMID:25826316</ref> <ref>PMID:25908396</ref> <ref>PMID:8119288</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n0/1n0i_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n0i ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1N0I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with CD, CL and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ferrochelatase Ferrochelatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.99.1.1 4.99.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1N0I OCA].
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*[[Ferrochelatase 3D structures|Ferrochelatase 3D structures]]
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== References ==
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==Reference==
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<references/>
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Metal binding to Bacillus subtilis ferrochelatase and interaction between metal sites., Lecerof D, Fodje MN, Alvarez Leon R, Olsson U, Hansson A, Sigfridsson E, Ryde U, Hansson M, Al-Karadaghi S, J Biol Inorg Chem. 2003 Apr;8(4):452-8. Epub 2003 Jan 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12761666 12761666]
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__TOC__
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Ferrochelatase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Al-Karadaghi S]]
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[[Category: Al-Karadaghi, S.]]
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[[Category: Fodje MN]]
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[[Category: Fodje, M.N.]]
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[[Category: Hansson A]]
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[[Category: Hansson, A.]]
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[[Category: Hansson M]]
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[[Category: Hansson, M.]]
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[[Category: Lecerof D]]
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[[Category: Lecerof, D.]]
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[[Category: Leon RA]]
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[[Category: Leon, R.A.]]
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[[Category: Olsson U]]
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[[Category: Olsson, U.]]
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[[Category: Ryde U]]
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[[Category: Ryde, U.]]
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[[Category: Sigfridsson E]]
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[[Category: Sigfridsson, E.]]
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[[Category: CD]]
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[[Category: CL]]
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[[Category: MG]]
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[[Category: pi-helix]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:51:17 2007''
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Current revision

Crystal Structure of Ferrochelatase with Cadmium bound at active site

PDB ID 1n0i

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