1n24

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(New page: 200px<br /><applet load="1n24" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n24, resolution 2.30&Aring;" /> '''(+)-Bornyl diphospha...)
Current revision (07:51, 14 February 2024) (edit) (undo)
 
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[[Image:1n24.gif|left|200px]]<br /><applet load="1n24" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1n24, resolution 2.30&Aring;" />
 
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'''(+)-Bornyl diphosphate synthase: Complex with Mg and product'''<br />
 
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==Overview==
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==(+)-Bornyl diphosphate synthase: Complex with Mg and product==
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The x-ray crystal structure of dimeric (+)-bornyl diphosphate synthase, a, metal-requiring monoterpene cyclase from Salvia officinalis, is reported, at 2.0-A resolution. Each monomer contains two alpha-helical domains: the, C-terminal domain catalyzes the cyclization of geranyl diphosphate, orienting and stabilizing multiple reactive carbocation intermediates; the, N-terminal domain has no clearly defined function, although its N terminus, caps the active site in the C-terminal domain during catalysis. Structures, of complexes with aza analogues of substrate and carbocation, intermediates, as well as complexes with pyrophosphate and bornyl, diphosphate, provide "snapshots" of the terpene cyclization cascade.
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<StructureSection load='1n24' size='340' side='right'caption='[[1n24]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1n24]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salvia_officinalis Salvia officinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N24 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N24 FirstGlance]. <br>
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1N24 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salvia_officinalis Salvia officinalis] with MG and BP2 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Geranyl-diphosphate_cyclase Geranyl-diphosphate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.8 5.5.1.8] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1N24 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BP2:(+)-BORNYL+DIPHOSPHATE'>BP2</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n24 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n24 OCA], [https://pdbe.org/1n24 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n24 RCSB], [https://www.ebi.ac.uk/pdbsum/1n24 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n24 ProSAT]</span></td></tr>
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Bornyl diphosphate synthase: structure and strategy for carbocation manipulation by a terpenoid cyclase., Whittington DA, Wise ML, Urbansky M, Coates RM, Croteau RB, Christianson DW, Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15375-80. Epub 2002 Nov 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12432096 12432096]
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</table>
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[[Category: Geranyl-diphosphate cyclase]]
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== Function ==
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[https://www.uniprot.org/uniprot/BPPS_SALOF BPPS_SALOF] Catalyzes the formation of the (+)-camphor precursor (+)-bornyl diphosphate from geranyl diphosphate. The enzyme also produces significant amounts of (+)-alpha-pinene, (+)-camphene, and (+-)-limonene.<ref>PMID:9614092</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n2/1n24_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n24 ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Salvia officinalis]]
[[Category: Salvia officinalis]]
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[[Category: Single protein]]
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[[Category: Christianson DW]]
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[[Category: Christianson, D.W.]]
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[[Category: Coates RM]]
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[[Category: Coates, R.M.]]
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[[Category: Croteau RB]]
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[[Category: Croteau, R.B.]]
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[[Category: Urbansky M]]
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[[Category: Urbansky, M.]]
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[[Category: Whittington DA]]
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[[Category: Whittington, D.A.]]
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[[Category: Wise ML]]
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[[Category: Wise, M.L.]]
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[[Category: BP2]]
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[[Category: MG]]
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[[Category: terpene synthase fold]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:53:06 2007''
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Current revision

(+)-Bornyl diphosphate synthase: Complex with Mg and product

PDB ID 1n24

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