1n38

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(New page: 200px<br /><applet load="1n38" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n38, resolution 2.8&Aring;" /> '''reovirus polymerase l...)
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[[Image:1n38.gif|left|200px]]<br /><applet load="1n38" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1n38, resolution 2.8&Aring;" />
 
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'''reovirus polymerase lambda3 elongation complex with one phosphodiester bond formed'''<br />
 
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==Overview==
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==reovirus polymerase lambda3 elongation complex with one phosphodiester bond formed==
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The reovirus polymerase and those of other dsRNA viruses function within, the confines of a protein capsid to transcribe the tightly packed dsRNA, genome segments. The crystal structure of the reovirus polymerase, lambda3, determined at 2.5 A resolution, shows a fingers-palm-thumb core, similar to those of other viral polymerases, surrounded by major N- and, C-terminal elaborations, which create a cage-like structure, with four, channels leading to the catalytic site. This "caged" polymerase has, allowed us to visualize the results of several rounds of RNA, polymerization directly in the crystals. A 5' cap binding site on the, surface of lambda3 suggests a template retention mechanism by which, attachment of the 5' end of the plus-sense strand facilitates insertion of, the 3' end of the minus-sense strand into the template channel.
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<StructureSection load='1n38' size='340' side='right'caption='[[1n38]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1n38]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mammalian_orthoreovirus_3_Dearing Mammalian orthoreovirus 3 Dearing]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N38 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N38 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CH1:3-DEOXY-CYTIDINE-5-TRIPHOSPHATE'>CH1</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=U3H:3-DEOXY-URIDINE+5-TRIPHOSPHATE'>U3H</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n38 OCA], [https://pdbe.org/1n38 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n38 RCSB], [https://www.ebi.ac.uk/pdbsum/1n38 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n38 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RDRP_REOVD RDRP_REOVD] RNA-directed RNA polymerase that is involved in transcription and genome replication. Following infection, it catalyzes the synthesis of fully conservative plus strands. After core assembly, which consists in recruitment of one capped plus-strand for each genomic segments and polymerase complexes, the polymerase switches mode and catalyzes the synthesis of complementary minus-strands.
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==About this Structure==
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==See Also==
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1N38 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mammalian_orthoreovirus_3 Mammalian orthoreovirus 3] with MN, U3H and CH1 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1N38 OCA].
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*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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RNA synthesis in a cage--structural studies of reovirus polymerase lambda3., Tao Y, Farsetta DL, Nibert ML, Harrison SC, Cell. 2002 Nov 27;111(5):733-45. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12464184 12464184]
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[[Category: Large Structures]]
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[[Category: Mammalian orthoreovirus 3]]
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[[Category: Mammalian orthoreovirus 3 Dearing]]
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[[Category: Single protein]]
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[[Category: Farsetta DL]]
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[[Category: Farsetta, D.L.]]
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[[Category: Harrison SC]]
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[[Category: Harrison, S.C.]]
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[[Category: Nibert ML]]
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[[Category: Nibert, M.L.]]
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[[Category: Tao Y]]
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[[Category: Tao, Y.]]
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[[Category: CH1]]
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[[Category: MN]]
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[[Category: U3H]]
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[[Category: bracelet]]
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[[Category: right hand configuration]]
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[[Category: rna polymerase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:55:35 2007''
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Current revision

reovirus polymerase lambda3 elongation complex with one phosphodiester bond formed

PDB ID 1n38

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