1xcp

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{{Seed}}
 
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[[Image:1xcp.png|left|200px]]
 
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==Crystal Structure of the Nitrogenase Fe protein Phe135Trp with MgADP bound==
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The line below this paragraph, containing "STRUCTURE_1xcp", creates the "Structure Box" on the page.
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<StructureSection load='1xcp' size='340' side='right'caption='[[1xcp]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1xcp]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XCP FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
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{{STRUCTURE_1xcp| PDB=1xcp | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xcp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xcp OCA], [https://pdbe.org/1xcp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xcp RCSB], [https://www.ebi.ac.uk/pdbsum/1xcp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xcp ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NIFH1_AZOVI NIFH1_AZOVI] The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein (component 2) and a component 1 which is either a molybdenum-iron protein, a vanadium-iron, or an iron-iron protein.[HAMAP-Rule:MF_00533]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xc/1xcp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xcp ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of the Azotobacter vinelandii nitrogenase Fe protein with phenylalanine at position 135 substituted by tryptophan has been determined in MgADP-bound form by X-ray diffraction methods. Amino acid substitution studies have suggested that the phenylalanine at position 135 located near the [4Fe-4S] cluster contributes to both the midpoint potential and nucleotide-induced changes of the [4Fe-4S] cluster. Substitution of tryptophan for phenylalanine at position 135 resulted in a significant positive shift in the midpoint potential in both the isolated and nucleotide-bound states. The factors thought to control the midpoint potential of the [FeS] cluster include solvent accessibility, dipolar environment, and structural strain. The structure derived in the present work provides an explanation for the more positive midpoint potential observed in the nucleotide-bound state, and suggests important insights into the contributions of the nucleotide interaction to the conformational states that are the keys to nitrogenase catalysis. The presence of MgADP in Phe135Trp Fe protein reveals the mechanism of the long-range communication from the nucleotide-binding site that controls its affinity for the MoFe protein component.
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===Crystal Structure of the Nitrogenase Fe protein Phe135Trp with MgADP bound===
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Structural basis for the changes in redox potential in the nitrogenase Phe135Trp Fe protein with MgADP Bound.,Jeong MS, Jang SB Mol Cells. 2004 Dec 31;18(3):374-82. PMID:15650336<ref>PMID:15650336</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1xcp" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_15650336}}, adds the Publication Abstract to the page
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*[[Nitrogenase 3D structures|Nitrogenase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 15650336 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15650336}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1XCP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCP OCA].
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==Reference==
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Structural basis for the changes in redox potential in the nitrogenase Phe135Trp Fe protein with MgADP Bound., Jeong MS, Jang SB, Mol Cells. 2004 Dec 31;18(3):374-82. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15650336 15650336]
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[[Category: Azotobacter vinelandii]]
[[Category: Azotobacter vinelandii]]
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[[Category: Nitrogenase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Jang SB]]
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[[Category: Jang, S B.]]
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[[Category: Jeong MS]]
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[[Category: Jeong, M S.]]
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[[Category: F135w]]
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[[Category: Fe protein]]
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[[Category: Mgadp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:22:37 2008''
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Current revision

Crystal Structure of the Nitrogenase Fe protein Phe135Trp with MgADP bound

PDB ID 1xcp

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