2f8q

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{{Seed}}
 
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[[Image:2f8q.png|left|200px]]
 
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==An alkali thermostable F/10 xylanase from alkalophilic Bacillus sp. NG-27==
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The line below this paragraph, containing "STRUCTURE_2f8q", creates the "Structure Box" on the page.
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<StructureSection load='2f8q' size='340' side='right'caption='[[2f8q]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2f8q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp._NG-27 Bacillus sp. NG-27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F8Q FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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{{STRUCTURE_2f8q| PDB=2f8q | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8q OCA], [https://pdbe.org/2f8q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f8q RCSB], [https://www.ebi.ac.uk/pdbsum/2f8q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f8q ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O30700_9BACI O30700_9BACI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f8/2f8q_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f8q ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Crystal structures are known for several glycosyl hydrolase family 10 (GH10) xylanases. However, none of them is from an alkalophilic organism that can grow in alkaline conditions. We have determined the crystal structures at 2.2 Angstroms of a GH10 extracellular endoxylanase (BSX) from an alkalophilic Bacillus sp. NG-27, for the native and the complex enzyme with xylosaccharides. The industrially important enzyme is optimally active and stable at 343 K and at a pH of 8.4. Comparison of the structure of BSX with those of other thermostable GH10 xylanases optimally active at acidic or close to neutral pH showed that the solvent-exposed acidic amino acids, Asp and Glu, are markedly enhanced in BSX, while solvent-exposed Asn was noticeably depleted. The BSX crystal structure when compared with putative three-dimensional homology models of other extracellular alkalophilic GH10 xylanases from alkalophilic organisms suggests that a protein surface rich in acidic residues may be an important feature common to these alkali thermostable enzymes. A comparison of the surface features of BSX and of halophilic proteins allowed us to predict the activity of BSX at high salt concentrations, which we verified through experiments. This offered us important lessons in the polyextremophilicity of proteins, where understanding the structural features of a protein stable in one set of extreme conditions provided clues about the activity of the protein in other extreme conditions. The work brings to the fore the role of the nature and composition of solvent-exposed residues in the adaptation of enzymes to polyextreme conditions, as in BSX.
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===An alkali thermostable F/10 xylanase from alkalophilic Bacillus sp. NG-27===
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Crystal structures of native and xylosaccharide-bound alkali thermostable xylanase from an alkalophilic Bacillus sp. NG-27: structural insights into alkalophilicity and implications for adaptation to polyextreme conditions.,Manikandan K, Bhardwaj A, Gupta N, Lokanath NK, Ghosh A, Reddy VS, Ramakumar S Protein Sci. 2006 Aug;15(8):1951-60. Epub 2006 Jul 5. PMID:16823036<ref>PMID:16823036</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_16823036}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2f8q" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 16823036 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16823036}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Bacillus sp. NG-27]]
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2F8Q is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8Q OCA].
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[[Category: Large Structures]]
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[[Category: Bhardwaj A]]
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==Reference==
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[[Category: Ghosh A]]
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Crystal structures of native and xylosaccharide-bound alkali thermostable xylanase from an alkalophilic Bacillus sp. NG-27: structural insights into alkalophilicity and implications for adaptation to polyextreme conditions., Manikandan K, Bhardwaj A, Gupta N, Lokanath NK, Ghosh A, Reddy VS, Ramakumar S, Protein Sci. 2006 Aug;15(8):1951-60. Epub 2006 Jul 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16823036 16823036]
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[[Category: Manikandan K]]
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[[Category: Bacteria]]
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[[Category: Ramakumar S]]
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[[Category: Endo-1,4-beta-xylanase]]
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[[Category: Reddy VS]]
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[[Category: Single protein]]
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[[Category: Bhardwaj, A.]]
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[[Category: Ghosh, A.]]
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[[Category: Manikandan, K.]]
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[[Category: Ramakumar, S.]]
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[[Category: Reddy, V S.]]
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[[Category: Alkali thermostable family 10 xylanase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:30:41 2008''
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Current revision

An alkali thermostable F/10 xylanase from alkalophilic Bacillus sp. NG-27

PDB ID 2f8q

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