2fzu

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{{Seed}}
 
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[[Image:2fzu.png|left|200px]]
 
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==Reduced enolate chromophore intermediate for GFP variant==
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The line below this paragraph, containing "STRUCTURE_2fzu", creates the "Structure Box" on the page.
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<StructureSection load='2fzu' size='340' side='right'caption='[[2fzu]], [[Resolution|resolution]] 1.25&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2fzu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FZU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FZU FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.25&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C12:2-(1-AMINO-2-HYDROXYPROPYL)-4-(4-HYDROXYBENZYL)-1-(2-OXOETHYL)-1H-IMIDAZOL-5-OLATE'>C12</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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{{STRUCTURE_2fzu| PDB=2fzu | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fzu OCA], [https://pdbe.org/2fzu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fzu RCSB], [https://www.ebi.ac.uk/pdbsum/2fzu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fzu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fz/2fzu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fzu ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Aequorea victoria green fluorescent protein (GFP) creates a fluorophore from its component amino acids Ser65, Tyr66, and Gly67 through a remarkable post-translational modification, involving spontaneous peptide backbone cyclization, dehydration, and oxidation reactions. Here we test and extend the understanding of fluorophore biosynthesis by coupling chemical reduction and anaerobic methodologies with kinetic analyses and protein structure determination. Two high-resolution structures of dithionite-treated GFP variants reveal a previously uncharacterized enolate intermediate form of the chromophore that is viable in generating a fluorophore (t1/2 = 39 min-1) upon exposure to air. Isolation of this enolate intermediate will now allow specific probing of the rate-limiting oxidation step for fluorophore biosynthesis in GFP and its red fluorescent protein homologues. Such targeted characterizations may lead to the design of faster maturing proteins with enhanced applications in biotechnology and cell biology. Moreover, our results reveal how the GFP protein environment mimics enzyme systems, by stabilizing an otherwise high energy enolate intermediate to achieve its post-translational modification.
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===Reduced enolate chromophore intermediate for GFP variant===
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Structural evidence for an enolate intermediate in GFP fluorophore biosynthesis.,Barondeau DP, Tainer JA, Getzoff ED J Am Chem Soc. 2006 Mar 15;128(10):3166-8. PMID:16522096<ref>PMID:16522096</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2fzu" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16522096}}, adds the Publication Abstract to the page
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16522096 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16522096}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2FZU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FZU OCA].
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==Reference==
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Structural evidence for an enolate intermediate in GFP fluorophore biosynthesis., Barondeau DP, Tainer JA, Getzoff ED, J Am Chem Soc. 2006 Mar 15;128(10):3166-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16522096 16522096]
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[[Category: Aequorea victoria]]
[[Category: Aequorea victoria]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Barondeau, D P.]]
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[[Category: Barondeau DP]]
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[[Category: Getzoff, E D.]]
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[[Category: Getzoff ED]]
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[[Category: Tainer, J A.]]
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[[Category: Tainer JA]]
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[[Category: Biosynthesis]]
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[[Category: Chromophore]]
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[[Category: High resolution]]
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[[Category: Intermediate]]
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[[Category: Post-translational modification]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:38:43 2008''
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Current revision

Reduced enolate chromophore intermediate for GFP variant

PDB ID 2fzu

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OCA

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