1n6m

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(New page: 200px<br /><applet load="1n6m" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n6m, resolution 2.5&Aring;" /> '''Rotation of the stalk...)
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[[Image:1n6m.gif|left|200px]]<br /><applet load="1n6m" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1n6m, resolution 2.5&Aring;" />
 
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'''Rotation of the stalk/neck and one head in a new crystal structure of the kinesin motor protein, Ncd'''<br />
 
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==Overview==
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==Rotation of the stalk/neck and one head in a new crystal structure of the kinesin motor protein, Ncd==
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Molecular motors undergo conformational changes to produce force and move, along cytoskeletal filaments. Structural changes have been detected in, kinesin motors; however, further changes are expected because previous, crystal structures are in the same or closely related conformations. We, report here a 2.5 A crystal structure of the minus-end kinesin, Ncd, with, the coiled-coil stalk/neck and one head rotated by approximately 75, degrees relative to the other head. The two heads are asymmetrically, positioned with respect to the stalk and show asymmetry of nucleotide, state: one head is fully occupied, but the other is unstably bound to ADP., Unlike previous structures, our new atomic model can be fit into, cryoelectron microscopy density maps of the motor attached to, microtubules, where it appears to resemble a one-head-bound motor with the, stalk rotated towards the minus end. Interactions between neck and motor, core residues, observed in the head that moves with the stalk, are, disrupted in the other head, permitting rotation of the stalk/neck. The, rotation could represent a force-producing stroke that directs the motor, to the minus end.
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<StructureSection load='1n6m' size='340' side='right'caption='[[1n6m]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1n6m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N6M FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n6m OCA], [https://pdbe.org/1n6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n6m RCSB], [https://www.ebi.ac.uk/pdbsum/1n6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n6m ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NCD_DROME NCD_DROME] NCD is required for normal chromosomal segregation in meiosis, in females, and in early mitotic divisions of the embryo. The NCD motor activity is directed toward the microtubule's minus end.<ref>PMID:2146510</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n6/1n6m_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n6m ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1N6M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with MG and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1N6M OCA].
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*[[Kinesin 3D Structures|Kinesin 3D Structures]]
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== References ==
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==Reference==
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<references/>
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Rotation of the stalk/neck and one head in a new crystal structure of the kinesin motor protein, Ncd., Yun M, Bronner CE, Park CG, Cha SS, Park HW, Endow SA, EMBO J. 2003 Oct 15;22(20):5382-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14532111 14532111]
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__TOC__
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</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bronner, C.E.]]
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[[Category: Bronner CE]]
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[[Category: Cha, S.S.]]
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[[Category: Cha S-S]]
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[[Category: Endow, S.A.]]
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[[Category: Endow SA]]
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[[Category: Park, C.G.]]
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[[Category: Park C-G]]
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[[Category: Park, H.W.]]
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[[Category: Park H-W]]
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[[Category: Yun, M.]]
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[[Category: Yun M]]
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[[Category: ADP]]
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[[Category: MG]]
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[[Category: kinesin]]
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[[Category: microtubule]]
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[[Category: motor]]
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[[Category: ncd]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:00:20 2007''
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Current revision

Rotation of the stalk/neck and one head in a new crystal structure of the kinesin motor protein, Ncd

PDB ID 1n6m

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