2qvw

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{{Seed}}
 
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[[Image:2qvw.png|left|200px]]
 
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==Structure of Giardia Dicer refined against twinned data==
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The line below this paragraph, containing "STRUCTURE_2qvw", creates the "Structure Box" on the page.
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<StructureSection load='2qvw' size='340' side='right'caption='[[2qvw]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2qvw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QVW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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{{STRUCTURE_2qvw| PDB=2qvw | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qvw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qvw OCA], [https://pdbe.org/2qvw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qvw RCSB], [https://www.ebi.ac.uk/pdbsum/2qvw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qvw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DCL_GIAIC DCL_GIAIC] Involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. It produces 21 to 23 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs.<ref>PMID:16410517</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of the enzyme Dicer from Giardia intestinalis was solved to 3.3 A resolution by MAD using crystals belonging to space group P2(1)2(1)2 [Macrae et al. (2006), Science, 311, 195-198]. These crystals were derived from crystals that diffracted X-rays to 3.0 A resolution but were refractory to structure determination because they were twinned. It is shown here that the original Dicer crystals represent an unusual case of perfect pseudo-merohedral twinning of orthorhombic crystals. Before the twinning problem was overcome, it was possible to calculate a low-resolution electron-density map in space group P4(1) that was used to build a partial molecular model. Experimental phases were sufficient to identify heavy-atom sites that indicated space-group inconsistency, leading to identification of the true space group. This information guided the search for different crystallization conditions that yielded untwinned crystals and ultimately a fully interpretable electron-density map.
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===Structure of Giardia Dicer refined against twinned data===
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An unusual case of pseudo-merohedral twinning in orthorhombic crystals of Dicer.,MacRae IJ, Doudna JA Acta Crystallogr D Biol Crystallogr. 2007 Sep;63(Pt 9):993-9. Epub 2007, Aug 17. PMID:17704568<ref>PMID:17704568</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17704568}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2qvw" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17704568 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17704568}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2QVW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVW OCA].
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==Reference==
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An unusual case of pseudo-merohedral twinning in orthorhombic crystals of Dicer., MacRae IJ, Doudna JA, Acta Crystallogr D Biol Crystallogr. 2007 Sep;63(Pt 9):993-9. Epub 2007, Aug 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17704568 17704568]
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[[Category: Giardia intestinalis]]
[[Category: Giardia intestinalis]]
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[[Category: Ribonuclease III]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Doudna JA]]
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[[Category: Doudna, J A.]]
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[[Category: MacRae IJ]]
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[[Category: MacRae, I J]]
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[[Category: Dicer]]
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[[Category: Hydrolase]]
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[[Category: Rnai,pseudo-merohedral twin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:54:25 2008''
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Current revision

Structure of Giardia Dicer refined against twinned data

PDB ID 2qvw

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