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1nf4

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(New page: 200px<br /><applet load="1nf4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nf4, resolution 2.05&Aring;" /> '''X-Ray Structure of t...)
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[[Image:1nf4.gif|left|200px]]<br /><applet load="1nf4" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1nf4, resolution 2.05&Aring;" />
 
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'''X-Ray Structure of the Desulfovibrio desulfuricans bacterioferritin: the diiron site in different states (reduced structure)'''<br />
 
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==Overview==
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==X-Ray Structure of the Desulfovibrio desulfuricans bacterioferritin: the diiron site in different states (reduced structure)==
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The first crystal structure of a native di-iron center in an iron-storage, protein (bacterio)ferritin is reported. The protein, isolated from the, anaerobic bacterium Desulfovibrio desulfuricans, has the unique property, of having Fe-coproporphyrin III as its heme cofactor. The, three-dimensional structure of this bacterioferritin was determined in, three distinct catalytic/redox states by X-ray crystallography (at 1.95, 2.05 and 2.35 A resolution), corresponding to different intermediates of, the di-iron ferroxidase site. Conformational changes associated with these, intermediates support the idea of a route for iron entry into the protein, shell through a pore that passes through the di-iron center. Molecular, surface and electrostatic potential calculations also suggest the presence, of another ion channel, distant from the channels at the three- and, four-fold axes proposed as points of entry for the iron atoms.
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<StructureSection load='1nf4' size='340' side='right'caption='[[1nf4]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1nf4]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfovibrio_desulfuricans Desulfovibrio desulfuricans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NF4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NF4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=FEC:1,3,5,8-TETRAMETHYL-PORPHINE-2,4,6,7-TETRAPROPIONIC+ACID+FERROUS+COMPLEX'>FEC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nf4 OCA], [https://pdbe.org/1nf4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nf4 RCSB], [https://www.ebi.ac.uk/pdbsum/1nf4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nf4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BFR_DESDA BFR_DESDA] Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nf/1nf4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nf4 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1NF4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_desulfuricans Desulfovibrio desulfuricans] with FE2, SO4 and FEC as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NF4 OCA].
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*[[Ferritin 3D structures|Ferritin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The nature of the di-iron site in the bacterioferritin from Desulfovibrio desulfuricans., Macedo S, Romao CV, Mitchell E, Matias PM, Liu MY, Xavier AV, LeGall J, Teixeira M, Lindley P, Carrondo MA, Nat Struct Biol. 2003 Apr;10(4):285-90. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12627224 12627224]
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[[Category: Desulfovibrio desulfuricans]]
[[Category: Desulfovibrio desulfuricans]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Carrondo, M.A.]]
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[[Category: Carrondo MA]]
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[[Category: LeGall, J.]]
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[[Category: LeGall J]]
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[[Category: Lindley, P.]]
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[[Category: Lindley P]]
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[[Category: Liu, M.Y.]]
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[[Category: Liu MY]]
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[[Category: Macedo, S.]]
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[[Category: Macedo S]]
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[[Category: Matias, P.M.]]
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[[Category: Matias PM]]
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[[Category: Mitchell, E.]]
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[[Category: Mitchell E]]
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[[Category: Romao, C.V.]]
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[[Category: Romao CV]]
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[[Category: Teixeira, M.]]
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[[Category: Teixeira M]]
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[[Category: Xavier, A.V.]]
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[[Category: Xavier AV]]
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[[Category: FE2]]
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[[Category: FEC]]
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[[Category: SO4]]
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[[Category: active as 24-mer]]
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[[Category: bacterioferritin]]
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[[Category: diiron centre]]
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[[Category: fe-coproporphyrin iii haem cofactor]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:12:27 2007''
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Current revision

X-Ray Structure of the Desulfovibrio desulfuricans bacterioferritin: the diiron site in different states (reduced structure)

PDB ID 1nf4

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