1ur6

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{{Seed}}
 
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[[Image:1ur6.png|left|200px]]
 
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==NMR based structural model of the UbcH5B-CNOT4 complex==
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The line below this paragraph, containing "STRUCTURE_1ur6", creates the "Structure Box" on the page.
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<StructureSection load='1ur6' size='340' side='right'caption='[[1ur6]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1ur6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UR6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UR6 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Hybrid , Solution NMR , Theoretical Model</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_1ur6| PDB=1ur6 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ur6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ur6 OCA], [https://pdbe.org/1ur6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ur6 RCSB], [https://www.ebi.ac.uk/pdbsum/1ur6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ur6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UB2D2_HUMAN UB2D2_HUMAN] Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection. Essential for viral activation of IRF3.<ref>PMID:10329681</ref> <ref>PMID:15280377</ref> <ref>PMID:18042044</ref> <ref>PMID:18703417</ref> <ref>PMID:18359941</ref> <ref>PMID:19854139</ref> <ref>PMID:20403326</ref> <ref>PMID:20061386</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ur/1ur6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ur6 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The protein CNOT4 possesses an N-terminal RING finger domain that acts as an E3 ubiquitin ligase and specifically interacts with UbcH5B, a ubiquitin-conjugating enzyme. The structure of the CNOT4 RING domain has been solved and the amino acids important for the binding to UbcH5B have been mapped. Here, the residues of UbcH5B important for the binding to CNOT4 RING domain were identified by NMR chemical shift perturbation experiments, and these data were used to generate structural models of the complex with the program HADDOCK. Together with the NMR data, additional biochemical data were included in a second docking, and comparisons of the resulting model with the structure of the c-Cbl/UbcH7 complex reveal some significant differences, notably at specific residues, and give structural insights into the E2/E3 specificity.
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===NMR BASED STRUCTURAL MODEL OF THE UBCH5B-CNOT4 COMPLEX===
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Structural model of the UbcH5B/CNOT4 complex revealed by combining NMR, mutagenesis, and docking approaches.,Dominguez C, Bonvin AM, Winkler GS, van Schaik FM, Timmers HT, Boelens R Structure. 2004 Apr;12(4):633-44. PMID:15062086<ref>PMID:15062086</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1ur6" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[3D structures of ubiquitin conjugating enzyme|3D structures of ubiquitin conjugating enzyme]]
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(as it appears on PubMed at http://www.pubmed.gov), where 15062086 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15062086}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1UR6 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UR6 OCA].
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==Reference==
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Structural model of the UbcH5B/CNOT4 complex revealed by combining NMR, mutagenesis, and docking approaches., Dominguez C, Bonvin AM, Winkler GS, van Schaik FM, Timmers HT, Boelens R, Structure. 2004 Apr;12(4):633-44. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15062086 15062086]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Ubiquitin--protein ligase]]
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[[Category: Boelens R]]
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[[Category: Boelens, R.]]
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[[Category: Bonvin AMJJ]]
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[[Category: Bonvin, A M.J J.]]
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[[Category: Dominguez C]]
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[[Category: Dominguez, C.]]
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[[Category: Timmers HThM]]
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[[Category: Schaik, F M.A Van.]]
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[[Category: Van Schaik FMA]]
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[[Category: Timmers, H Th M.]]
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[[Category: Winkler GS]]
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[[Category: Winkler, G S.]]
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[[Category: Ccr4-not complex]]
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[[Category: Ligase]]
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[[Category: Ring finger protein]]
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[[Category: Transcription regulation]]
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[[Category: Ubiquitin conjugating enzyme]]
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[[Category: Ubiquitin ligase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:10:57 2008''
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Current revision

NMR based structural model of the UbcH5B-CNOT4 complex

PDB ID 1ur6

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