2pk9

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{{Seed}}
 
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[[Image:2pk9.png|left|200px]]
 
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==Structure of the Pho85-Pho80 CDK-cyclin Complex of the Phosphate-responsive Signal Transduction Pathway==
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The line below this paragraph, containing "STRUCTURE_2pk9", creates the "Structure Box" on the page.
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<StructureSection load='2pk9' size='340' side='right'caption='[[2pk9]], [[Resolution|resolution]] 2.91&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2pk9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PK9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PK9 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.906&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
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{{STRUCTURE_2pk9| PDB=2pk9 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pk9 OCA], [https://pdbe.org/2pk9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pk9 RCSB], [https://www.ebi.ac.uk/pdbsum/2pk9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pk9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PHO85_YEAST PHO85_YEAST] Cyclin-dependent protein kinase (CDK) catalytic subunit that regulates multiple cell cycle and metabolic processes in response to nutrient availability. Associates with different cyclins, that control kinase activity, substrate-specificity and subcellular location of the kinase. Favorable growth conditions always result in activated cyclin-CDK complexes. Regulates metabolic processes when associated with PHO80 cyclin family members (PH080, PCL6, PCL7, PCL8 and PCL10), and cell cycle and morphogenesis processes when associated with PCL1,2 cyclin family members (PCL1, PCL2, CLG1, PCL5 and PCL9). When associated with PHO80, negatively regulates the expression of phosphate-starvation-responsive genes under phosphate-rich conditions. The PHO80-PHO85 cyclin-CDK holoenzyme phosphorylates and inactivates the transcription factor PHO4 by promoting its export to the cytoplasm. PHO80-PHO85 phosphorylates and inactivates protein kinase RIM15 by retaining it in the cytoplasm, antagonizing RIM15-induced entry into stationary phase. PHO80-PHO85 also phosphorylates and inactivates the calcineurin-responsive transcription factor CRZ1, linking cyclin-CDK activity to calcium signaling. Together with the cyclins PCL6/PCL7 and PCL8/PCL10, negatively controls glycogen accumulation. When associated with cyclins PCL6 and PCL7, controls glycogen phosphorylase and glycogen synthase activities. PCL6-PHO85 and PCL7-PHO85 phosphorylate and inactivate the phosphatase PP1-2 inhibitor GLC8, causing activation of PP1-2, which then dephosphorylates and activates glycogen phosphorylase. When associated with cyclins PCL8 and PCL10, has glycogen synthase kinase activity. PCL10-PHO85 phosphorylates and negatively regulates glycogen synthase GSY2. Association with PCL1 and PCL2 is required for cell cycle progression at start in the absence of the CDC28-dependent G1 cyclins CLN1 and CLN2. PCL1-PHO85 is involved in phosphorylation of the CDK inhibitor (CKI) SIC1, which is required for its ubiquitination and degradation, releasing repression of b-type cyclins and promoting exit from mitosis. When associated with cyclins PCL1 and PCL2, positively controls degradation of sphingoid long chain base kinase LCB4 via phosphorylation of LCB4, which is required for its ubiquitination and degradation. PCL1-PHO85 also phosphorylates HMS1, NCP1 and NPA3, which may all have a role in mitotic exit. PCL2-PHO85 also phosphorylates RVS167, linking cyclin-CDK activity with organization of the actin cytoskeleton. When associated with PCL5, positively controls degradation of transcription factor GCN4 via phosphorylation of GCN4, which is required for its degradation by the E3 ubiquitin ligase complex SCF(Cdc4). When associated with PCL9, may have a role in bud site selection in G1 phase. PHO85 also phosphorylates the transcription factor SWI5.<ref>PMID:3067079</ref> <ref>PMID:8108735</ref> <ref>PMID:7973730</ref> <ref>PMID:8539622</ref> <ref>PMID:9843683</ref> <ref>PMID:9725902</ref> <ref>PMID:9584169</ref> <ref>PMID:9593297</ref> <ref>PMID:10490639</ref> <ref>PMID:10692159</ref> <ref>PMID:11602261</ref> <ref>PMID:12006994</ref> <ref>PMID:12098764</ref> <ref>PMID:12101234</ref> <ref>PMID:12407105</ref> <ref>PMID:12857883</ref> <ref>PMID:15057567</ref> <ref>PMID:15082539</ref> <ref>PMID:15721288</ref> <ref>PMID:16308562</ref> <ref>PMID:15598647</ref> <ref>PMID:16455487</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pk/2pk9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pk9 ConSurf].
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<div style="clear:both"></div>
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===Structure of the Pho85-Pho80 CDK-cyclin Complex of the Phosphate-responsive Signal Transduction Pathway===
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==See Also==
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*[[Cyclin 3D structures|Cyclin 3D structures]]
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*[[Pho4|Pho4]]
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== References ==
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<references/>
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(as it appears on PubMed at http://www.pubmed.gov), where 18042456 is the PubMed ID number.
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__TOC__
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</StructureSection>
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{{ABSTRACT_PUBMED_18042456}}
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[[Category: Large Structures]]
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==About this Structure==
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2PK9 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PK9 OCA].
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==Reference==
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Structure of the Pho85-Pho80 CDK-cyclin complex of the phosphate-responsive signal transduction pathway., Huang K, Ferrin-O'Connell I, Zhang W, Leonard GA, O'Shea EK, Quiocho FA, Mol Cell. 2007 Nov 30;28(4):614-23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18042456 18042456]
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[[Category: Cyclin-dependent kinase]]
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[[Category: Protein complex]]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Connell, I Ferrin-O.]]
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[[Category: Ferrin-O'Connell I]]
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[[Category: Huang, K.]]
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[[Category: Huang K]]
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[[Category: Leonard, G A.]]
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[[Category: Leonard GA]]
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[[Category: Quiocho, F A.]]
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[[Category: O'Shea EK]]
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[[Category: Shea, E K.O.]]
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[[Category: Quiocho FA]]
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[[Category: Zhang, W.]]
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[[Category: Zhang W]]
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[[Category: Cyclin]]
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[[Category: Cyclin-dependent kinase]]
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[[Category: Signaling protein]]
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[[Category: Signaling protein,transferase/cell cycle complex]]
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[[Category: Transferase/ cell cycle complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:23:24 2008''
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Current revision

Structure of the Pho85-Pho80 CDK-cyclin Complex of the Phosphate-responsive Signal Transduction Pathway

PDB ID 2pk9

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