1zgo

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{{Seed}}
 
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[[Image:1zgo.png|left|200px]]
 
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==High Resolution Crystal Structure of the Discosoma Red Fluorescent Protein (DsRed)==
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The line below this paragraph, containing "STRUCTURE_1zgo", creates the "Structure Box" on the page.
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<StructureSection load='1zgo' size='340' side='right'caption='[[1zgo]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1zgo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Discosoma_sp. Discosoma sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZGO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZGO FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr>
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{{STRUCTURE_1zgo| PDB=1zgo | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zgo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zgo OCA], [https://pdbe.org/1zgo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zgo RCSB], [https://www.ebi.ac.uk/pdbsum/1zgo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zgo ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RFP_DISSP RFP_DISSP] Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts.<ref>PMID:10504696</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zg/1zgo_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zgo ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The mature self-synthesizing p-hydroxybenzylideneimidazolinone-like fluorophores of Discosoma red fluorescent protein (DsRed) and Aequorea victoria green fluorescent protein (GFP) are extensively studied as powerful biological markers. Yet, the spontaneous formation of these fluorophores by cyclization, oxidation, and dehydration reactions of tripeptides within their protein environment remains incompletely understood. The mature DsRed fluorophore (Gln 66, Tyr 67, and Gly 68) differs from the GFP fluorophore by an acylimine that results in Gln 66 Calpha planar geometry and by a Phe 65-Gln 66 cis peptide bond. DsRed green-to-red maturation includes a green-fluorescing immature chromophore and requires a chromophore peptide bond trans-cis isomerization that is slow and incomplete. To clarify the unique structural chemistry for the individual immature "green" and mature "red" chromophores of DsRed, we report here the determination and analysis of crystal structures for the wild-type protein (1.4 A resolution), the entirely green DsRed K70M mutant protein (1.9 A resolution), and the DsRed designed mutant Q66M (1.9 A resolution), which shows increased red chromophore relative to the wild-type DsRed. Whereas the mature, red-fluorescing chromophore has the expected cis peptide bond and a sp(2)-hybridized Gln 66 Calpha with planar geometry, the crystal structure of the immature green-fluorescing chromophore of DsRed, presented here for the first time, reveals a trans peptide bond and a sp(3)-hybridized Gln 66 Calpha with tetrahedral geometry. These results characterize a GFP-like immature green DsRed chromophore structure, reveal distinct mature and immature chromophore environments, and furthermore provide evidence for the coupling of acylimine formation with trans-cis isomerization.
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===High Resolution Crystal Structure of the Discosoma Red Fluorescent Protein (DsRed)===
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Crystallographic structures of Discosoma red fluorescent protein with immature and mature chromophores: linking peptide bond trans-cis isomerization and acylimine formation in chromophore maturation.,Tubbs JL, Tainer JA, Getzoff ED Biochemistry. 2005 Jul 26;44(29):9833-40. PMID:16026155<ref>PMID:16026155</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1zgo" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16026155}}, adds the Publication Abstract to the page
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16026155 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16026155}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Discosoma sp]]
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1ZGO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Discosoma_sp. Discosoma sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZGO OCA].
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[[Category: Large Structures]]
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[[Category: Getzoff ED]]
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==Reference==
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[[Category: Tainer JA]]
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Crystallographic structures of Discosoma red fluorescent protein with immature and mature chromophores: linking peptide bond trans-cis isomerization and acylimine formation in chromophore maturation., Tubbs JL, Tainer JA, Getzoff ED, Biochemistry. 2005 Jul 26;44(29):9833-40. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16026155 16026155]
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[[Category: Tubbs JL]]
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[[Category: Discosoma sp.]]
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[[Category: Single protein]]
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[[Category: Getzoff, E D.]]
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[[Category: Tainer, J A.]]
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[[Category: Tubbs, J L.]]
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[[Category: Beta barrel]]
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[[Category: Beta can]]
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[[Category: Chromophore]]
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[[Category: Coral]]
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[[Category: Drfp583]]
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[[Category: Dsred]]
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[[Category: Fluorescent protein]]
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[[Category: Gfp]]
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[[Category: Red]]
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[[Category: Rfp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:36:01 2008''
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Current revision

High Resolution Crystal Structure of the Discosoma Red Fluorescent Protein (DsRed)

PDB ID 1zgo

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