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1njq

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(New page: 200px<br /><applet load="1njq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1njq" /> '''NMR structure of the single QALGGH zinc fing...)
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[[Image:1njq.gif|left|200px]]<br /><applet load="1njq" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1njq" />
 
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'''NMR structure of the single QALGGH zinc finger domain from Arabidopsis thaliana SUPERMAN protein'''<br />
 
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==Overview==
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==NMR structure of the single QALGGH zinc finger domain from Arabidopsis thaliana SUPERMAN protein==
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Zinc finger domains of the classical type represent the most abundant DNA, binding domains in eukaryotic transcription factors. Plant proteins, contain from one to four zinc finger domains, which are characterized by, high conservation of the sequence QALGGH, shown to be critical for, DNA-binding activity. The Arabidopsis thaliana SUPERMAN protein, which, contains a single QALGGH zinc finger, is necessary for proper spatial, development of reproductive floral tissues and has been shown to, specifically bind to DNA. Here, we report the synthesis and UV and NMR, spectroscopic structural characterization of a 37 amino acid SUPERMAN, region complexed to a Zn(2+) ion (Zn-SUP37) and present the first, high-resolution structure of a classical zinc finger domain from a plant, protein. The NMR structure of the SUPERMAN zinc finger domain consists of, a very well-defined betabetaalpha motif, typical of all other, Cys(2)-His(2) zinc fingers structurally characterized. As a consequence, the highly conserved QALGGH sequence is located at the N terminus of the, alpha helix. This region of the domain of animal zinc finger proteins, consists of hypervariable residues that are responsible for recognizing, the DNA bases. Therefore, we propose a peculiar DNA recognition code for, the QALGGH zinc finger domain that includes all or some of the amino acid, residues at positions -1, 2, and 3 (numbered relative to the N terminus of, the helix) and possibly others at the C-terminal end of the recognition, helix. This study further confirms that the zinc finger domain, though, very simple, is an extremely versatile DNA binding motif.
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<StructureSection load='1njq' size='340' side='right'caption='[[1njq]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1njq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NJQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NJQ FirstGlance]. <br>
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1NJQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with ZN, ACE and NH2 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NJQ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1njq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1njq OCA], [https://pdbe.org/1njq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1njq RCSB], [https://www.ebi.ac.uk/pdbsum/1njq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1njq ProSAT]</span></td></tr>
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NMR structure of the single QALGGH zinc finger domain from the Arabidopsis thaliana SUPERMAN protein., Isernia C, Bucci E, Leone M, Zaccaro L, Di Lello P, Digilio G, Esposito S, Saviano M, Di Blasio B, Pedone C, Pedone PV, Fattorusso R, Chembiochem. 2003 Mar 3;4(2-3):171-80. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12616630 12616630]
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</table>
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[[Category: Single protein]]
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== Function ==
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[[Category: Blasio, B.Di.]]
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[https://www.uniprot.org/uniprot/SUP_ARATH SUP_ARATH] Probable transcriptional regulator considered as cadastral protein that acts indirectly to prevent the B class homeotic proteins APETALA3 and perhaps PISTILLATA from acting in the gynoecial whorl. Principal function is to balance cell proliferation in the third and fourth whorls of developing flowers thereby maintaining the boundary between stamens and carpels. May fulfill this role by repressing genes implicated in cell division. Plays equally a role in the determinacy of the floral meristem. Is also required for normal ovule development.<ref>PMID:7477325</ref> <ref>PMID:12324589</ref> <ref>PMID:1352237</ref> <ref>PMID:11006335</ref> <ref>PMID:11943180</ref>
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[[Category: Bucci, E.]]
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== Evolutionary Conservation ==
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[[Category: Digilio, G.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Esposito, S.]]
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Check<jmol>
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[[Category: Fattorusso, R.]]
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<jmolCheckbox>
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[[Category: Isernia, C.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nj/1njq_consurf.spt"</scriptWhenChecked>
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[[Category: Lello, P.Di.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: Leone, M.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: Pedone, C.]]
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</jmolCheckbox>
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[[Category: Pedone, P.V.]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1njq ConSurf].
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[[Category: Saviano, M.]]
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<div style="clear:both"></div>
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[[Category: Zaccaro, L.]]
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== References ==
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[[Category: ACE]]
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<references/>
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[[Category: NH2]]
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__TOC__
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[[Category: ZN]]
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</StructureSection>
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[[Category: beta-beta-alfa motif]]
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[[Category: Arabidopsis thaliana]]
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[[Category: peptide-zinc complex]]
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[[Category: Large Structures]]
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[[Category: zinc-finger]]
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[[Category: Bucci E]]
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[[Category: Di Blasio B]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:19:34 2007''
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[[Category: Di Lello P]]
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[[Category: Digilio G]]
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[[Category: Esposito S]]
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[[Category: Fattorusso R]]
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[[Category: Isernia C]]
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[[Category: Leone M]]
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[[Category: Pedone C]]
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[[Category: Pedone PV]]
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[[Category: Saviano M]]
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[[Category: Zaccaro L]]

Current revision

NMR structure of the single QALGGH zinc finger domain from Arabidopsis thaliana SUPERMAN protein

PDB ID 1njq

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