1nmf

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(New page: 200px<br /><applet load="1nmf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nmf" /> '''MAJOR COLD-SHOCK PROTEIN, NMR, 20 STRUCTURES...)
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[[Image:1nmf.jpg|left|200px]]<br /><applet load="1nmf" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1nmf" />
 
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'''MAJOR COLD-SHOCK PROTEIN, NMR, 20 STRUCTURES'''<br />
 
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==Overview==
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==MAJOR COLD-SHOCK PROTEIN, NMR, 20 STRUCTURES==
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The cold-shock domain (CSD) is found in many eukaryotic transcriptional, factors and is responsible for the specific binding to DNA of a, cis-element called the Y-box. The same domain exists in the sequence of, the Xenopus RNA-binding proteins FRG Y1 and FRG Y2 (refs 1, 3). The major, cold-shock proteins of Escherichia coli (CS7.4) and B. subtilis (CspB), have sequences that are more than 40 per cent identical to the cold-shock, domain. We present here the three-dimensional structure of CspB determined, by nuclear magnetic resonance spectroscopy. The 67-residue protein, consists of an antiparallel five-stranded beta-barrel with strands, connected by turns and loops. The structure resembles that of, staphylococcal nuclease and the gene-5 single-stranded-DNA-binding, protein. A three-stranded beta-sheet, which contains the conserved, RNA-binding motif RNP1 as well as a motif similar to RNP2 in two, neighbouring antiparallel beta-strands, has basic and aromatic residues at, its surface which could serve as a binding site for single-stranded DNA., CspB binds to single-stranded DNA in gel retardation experiments.
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<StructureSection load='1nmf' size='340' side='right'caption='[[1nmf]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1nmf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NMF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NMF FirstGlance]. <br>
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1NMF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NMF OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nmf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nmf OCA], [https://pdbe.org/1nmf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nmf RCSB], [https://www.ebi.ac.uk/pdbsum/1nmf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nmf ProSAT]</span></td></tr>
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==Reference==
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</table>
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Structure in solution of the major cold-shock protein from Bacillus subtilis., Schnuchel A, Wiltscheck R, Czisch M, Herrler M, Willimsky G, Graumann P, Marahiel MA, Holak TA, Nature. 1993 Jul 8;364(6433):169-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8321289 8321289]
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== Function ==
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[https://www.uniprot.org/uniprot/CSPB_BACSU CSPB_BACSU] Binds to the pentamer sequences ATTGG and CCAAT with highest affinity in single-stranded DNA, and also to other sequences. Has greater affinity for ATTGG than CCAAT. Can act as transcriptional activator of cold shock genes by recognizing putative ATTGG-box elements present in promoter regions of genes induced under cold shock conditions.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nm/1nmf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nmf ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Holak, T.A.]]
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[[Category: Holak TA]]
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[[Category: Schnuchel, A.]]
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[[Category: Schnuchel A]]
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[[Category: cold shock protein]]
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[[Category: transcription regulation]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:23:19 2007''
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MAJOR COLD-SHOCK PROTEIN, NMR, 20 STRUCTURES

PDB ID 1nmf

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