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1nne

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(New page: 200px<br /><applet load="1nne" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nne, resolution 3.11&Aring;" /> '''Crystal Structure of...)
Current revision (07:58, 14 February 2024) (edit) (undo)
 
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[[Image:1nne.gif|left|200px]]<br /><applet load="1nne" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1nne, resolution 3.11&Aring;" />
 
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'''Crystal Structure of the MutS-ADPBeF3-DNA complex'''<br />
 
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==Overview==
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==Crystal Structure of the MutS-ADPBeF3-DNA complex==
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During mismatch repair ATP binding and hydrolysis activities by the MutS, family proteins are important for both mismatch recognition and for, transducing mismatch recognition signals to downstream repair factors., Despite intensive efforts, a MutS.ATP.DNA complex has eluded, crystallographic analysis. Searching for ATP analogs that strongly bound, to Thermus aquaticus (Taq) MutS, we found that ADP.beryllium fluoride, (ABF), acted as a strong inhibitor of several MutS family ATPases., Furthermore, ABF promoted the formation of a ternary complex containing, the Saccharomyces cerevisiae MSH2.MSH6 and MLH1.PMS1 proteins bound to, mismatch DNA but did not promote dissociation of MSH2.MSH6 from mismatch, DNA. Crystallographic analysis of the Taq MutS.DNA.ABF complex indicated, that although this complex was very similar to that of MutS.DNA.ADP, both, ADP.Mg(2+) moieties in the MutS. DNA.ADP structure were replaced by ABF., Furthermore, a disordered region near the ATP-binding pocket in the MutS B, subunit became traceable, whereas the equivalent region in the A subunit, that interacts with the mismatched nucleotide remained disordered., Finally, the DNA binding domains of MutS together with the mismatched DNA, were shifted upon binding of ABF. We hypothesize that the presence of ABF, is communicated between the two MutS subunits through the contact between, the ordered loop and Domain III in addition to the intra-subunit helical, lever arm that links the ATPase and DNA binding domains.
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<StructureSection load='1nne' size='340' side='right'caption='[[1nne]], [[Resolution|resolution]] 3.11&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1nne]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NNE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NNE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.11&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nne FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nne OCA], [https://pdbe.org/1nne PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nne RCSB], [https://www.ebi.ac.uk/pdbsum/1nne PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nne ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MUTS_THEAQ MUTS_THEAQ] This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nn/1nne_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nne ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1NNE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus] with SO4, BEF, ADP and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NNE OCA].
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*[[DNA mismatch repair protein 3D structures|DNA mismatch repair protein 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure and biochemical analysis of the MutS.ADP.beryllium fluoride complex suggests a conserved mechanism for ATP interactions in mismatch repair., Alani E, Lee JY, Schofield MJ, Kijas AW, Hsieh P, Yang W, J Biol Chem. 2003 May 2;278(18):16088-94. Epub 2003 Feb 11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12582174 12582174]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Thermus aquaticus]]
[[Category: Thermus aquaticus]]
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[[Category: Alani, E.]]
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[[Category: Alani E]]
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[[Category: Hsieh, P.]]
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[[Category: Hsieh P]]
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[[Category: Kijas, A.W.]]
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[[Category: Kijas AW]]
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[[Category: Lee, J.Y.]]
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[[Category: Lee JY]]
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[[Category: Schofield, M.J.]]
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[[Category: Schofield MJ]]
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[[Category: Yang, W.]]
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[[Category: Yang W]]
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[[Category: ADP]]
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[[Category: BEF]]
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[[Category: EDO]]
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[[Category: SO4]]
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[[Category: dna]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:24:34 2007''
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Current revision

Crystal Structure of the MutS-ADPBeF3-DNA complex

PDB ID 1nne

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