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2z8g

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{{Seed}}
 
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[[Image:2z8g.png|left|200px]]
 
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<!--
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==Aspergillus niger ATCC9642 isopullulanase complexed with isopanose==
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The line below this paragraph, containing "STRUCTURE_2z8g", creates the "Structure Box" on the page.
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<StructureSection load='2z8g' size='340' side='right'caption='[[2z8g]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2z8g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z8G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z8G FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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{{STRUCTURE_2z8g| PDB=2z8g | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z8g OCA], [https://pdbe.org/2z8g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z8g RCSB], [https://www.ebi.ac.uk/pdbsum/2z8g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z8g ProSAT]</span></td></tr>
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</table>
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===Aspergillus niger ATCC9642 isopullulanase complexed with isopanose===
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== Function ==
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[https://www.uniprot.org/uniprot/IPUA_ASPNG IPUA_ASPNG] Hydrolyzes pullulan, a linear polymer which is composed of maltotriose units with alpha-1,6 glucosidic linkages, to produce isopanose (Glca1-4Glca1-6Glc).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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The line below this paragraph, {{ABSTRACT_PUBMED_18155243}}, adds the Publication Abstract to the page
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Check<jmol>
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(as it appears on PubMed at http://www.pubmed.gov), where 18155243 is the PubMed ID number.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z8/2z8g_consurf.spt"</scriptWhenChecked>
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{{ABSTRACT_PUBMED_18155243}}
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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2Z8G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z8G OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z8g ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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Crystal structure of Aspergillus niger isopullulanase, a member of glycoside hydrolase family 49., Mizuno M, Koide A, Yamamura A, Akeboshi H, Yoshida H, Kamitori S, Sakano Y, Nishikawa A, Tonozuka T, J Mol Biol. 2008 Feb 8;376(1):210-20. Epub 2007 Dec 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18155243 18155243]
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</StructureSection>
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Insights into the reaction mechanism of glycosyl hydrolase family 49. Site-directed mutagenesis and substrate preference of isopullulanase., Akeboshi H, Tonozuka T, Furukawa T, Ichikawa K, Aoki H, Shimonishi A, Nishikawa A, Sakano Y, Eur J Biochem. 2004 Nov;271(22):4420-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15560783 15560783]
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Construction of an efficient expression system for Aspergillus isopullulanase in Pichia pastoris, and a simple purification method., Akeboshi H, Kashiwagi Y, Aoki H, Tonozuka T, Nishikawa A, Sakano Y, Biosci Biotechnol Biochem. 2003 May;67(5):1149-53. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12834298 12834298]
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[[Category: Aspergillus niger]]
[[Category: Aspergillus niger]]
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[[Category: Isopullulanase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Akeboshi H]]
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[[Category: Akeboshi, H.]]
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[[Category: Kamitori S]]
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[[Category: Kamitori, S.]]
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[[Category: Koide A]]
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[[Category: Koide, A.]]
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[[Category: Mizuno M]]
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[[Category: Mizuno, M.]]
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[[Category: Nishikawa A]]
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[[Category: Nishikawa, A.]]
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[[Category: Sakano Y]]
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[[Category: Sakano, Y.]]
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[[Category: Tonozuka T]]
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[[Category: Tonozuka, T.]]
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[[Category: Yamamura A]]
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[[Category: Yamamura, A.]]
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[[Category: Yoshida H]]
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[[Category: Yoshida, H.]]
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[[Category: Beta-helix]]
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[[Category: Dextranase]]
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[[Category: Gh49]]
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[[Category: Glycoprotein]]
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[[Category: Glycosidase]]
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[[Category: Hydrolase]]
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[[Category: Isopullulanase]]
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[[Category: Pullulan]]
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[[Category: Secreted]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:19:50 2008''
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Current revision

Aspergillus niger ATCC9642 isopullulanase complexed with isopanose

PDB ID 2z8g

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