1xsk

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{{Seed}}
 
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[[Image:1xsk.png|left|200px]]
 
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==Structure of a Family 31 alpha glycosidase glycosyl-enzyme intermediate==
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The line below this paragraph, containing "STRUCTURE_1xsk", creates the "Structure Box" on the page.
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<StructureSection load='1xsk' size='340' side='right'caption='[[1xsk]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1xsk]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XSK FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPO:3[N-MORPHOLINO]PROPANE+SULFONIC+ACID'>MPO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XYF:5(R)-5-FLUORO-BETA-D-XYLOPYRANOSYL-ENZYME+INTERMEDIATE'>XYF</scene></td></tr>
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{{STRUCTURE_1xsk| PDB=1xsk | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xsk OCA], [https://pdbe.org/1xsk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xsk RCSB], [https://www.ebi.ac.uk/pdbsum/1xsk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xsk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/XYLS_ECOLI XYLS_ECOLI] Can catalyze the transfer of alpha-xylosyl residue from alpha-xyloside to xylose, glucose, mannose, fructose, maltose, isomaltose, nigerose, kojibiose, sucrose and trehalose.<ref>PMID:15294295</ref> <ref>PMID:15501829</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xs/1xsk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xsk ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We have determined the first structure of a family 31 alpha-glycosidase, that of YicI from Escherichia coli, both free and trapped as a 5-fluoroxylopyranosyl-enzyme intermediate via reaction with 5-fluoro-alpha-D-xylopyranosyl fluoride. Our 2.2-A resolution structure shows an intimately associated hexamer with structural elements from several monomers converging at each of the six active sites. Our kinetic and mass spectrometry analyses verified several of the features observed in our structural data, including a covalent linkage from the carboxylate side chain of the identified nucleophile Asp(416) to C-1 of the sugar ring. Structure-based sequence comparison of YicI with the mammalian alpha-glucosidases lysosomal alpha-glucosidase and sucrase-isomaltase predicts a high level of structural similarity and provides a foundation for understanding the various mutations of these enzymes that elicit human disease.
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===Structure of a Family 31 alpha glycosidase glycosyl-enzyme intermediate===
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Mechanistic and structural analysis of a family 31 alpha-glycosidase and its glycosyl-enzyme intermediate.,Lovering AL, Lee SS, Kim YW, Withers SG, Strynadka NC J Biol Chem. 2005 Jan 21;280(3):2105-15. Epub 2004 Oct 22. PMID:15501829<ref>PMID:15501829</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1xsk" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_15501829}}, adds the Publication Abstract to the page
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*[[Xylosidase 3D structures|Xylosidase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 15501829 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15501829}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1XSK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSK OCA].
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==Reference==
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Mechanistic and structural analysis of a family 31 alpha-glycosidase and its glycosyl-enzyme intermediate., Lovering AL, Lee SS, Kim YW, Withers SG, Strynadka NC, J Biol Chem. 2005 Jan 21;280(3):2105-15. Epub 2004 Oct 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15501829 15501829]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Kim, Y W.]]
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[[Category: Kim YW]]
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[[Category: Lee, S S.]]
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[[Category: Lee SS]]
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[[Category: Lovering, A L.]]
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[[Category: Lovering AL]]
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[[Category: Strynadka, N C.]]
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[[Category: Strynadka NC]]
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[[Category: Withers, S G.]]
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[[Category: Withers SG]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:36:41 2008''
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Current revision

Structure of a Family 31 alpha glycosidase glycosyl-enzyme intermediate

PDB ID 1xsk

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