1ueo

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{{Seed}}
 
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[[Image:1ueo.png|left|200px]]
 
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==Solution structure of the [T8A]-Penaeidin-3==
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The line below this paragraph, containing "STRUCTURE_1ueo", creates the "Structure Box" on the page.
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<StructureSection load='1ueo' size='340' side='right'caption='[[1ueo]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1ueo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Penaeus_vannamei Penaeus vannamei]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UEO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UEO FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ueo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ueo OCA], [https://pdbe.org/1ueo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ueo RCSB], [https://www.ebi.ac.uk/pdbsum/1ueo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ueo ProSAT]</span></td></tr>
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{{STRUCTURE_1ueo| PDB=1ueo | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PEN3A_PENVA PEN3A_PENVA] Antibacterial activity against M.luteus and E.coli bacteria. Antifungal activity against N.crassa and F.oxysporum. Presents chitin-binding activity.<ref>PMID:9353298</ref> <ref>PMID:10561573</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ue/1ueo_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ueo ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Penaeidins are a family of antimicrobial peptides of 47-63 residues isolated from several species of shrimp. These peptides display a proline-rich domain (N-terminal part) and a cysteine-rich domain (C-terminal part) stabilized by three conserved disulfide bonds whose arrangement has not yet been characterized. The recombinant penaeidin-3a of Litopenaeus vannamei (63 residues) and its [T8A]-Pen-3a analogue were produced in Saccharomyces cerevisiae and showed similar antimicrobial activity. The solution structure of the [T8A]-Pen-3a analogue was determined by using two-dimensional 1H NMR and simulated annealing calculations. The proline-rich domain, spanning residues 1-28 was found to be unconstrained. In contrast, the cysteine-rich domain, spanning residues 29-58, displays a well defined structure, which consists of an amphipathic helix (41-50) linked to the upstream and the downstream coils by two disulfide bonds (Cys32-Cys47 and Cys48-Cys55). These two coils are in turn linked together by the third disulfide bond (Cys36-Cys54). Such a disulfide bond packing, which is in agreement with the analysis of trypsin digests by ESI-MS, contributes to the highly hydrophobic core. Side chains of Arg45 and Arg50, which belong to the helix, and side chains of Arg37 and Arg53, which belong to the upstream and the downstream coils, are located in two opposite parts of this globular and compact structure. The environment of these positively charged residues, either by hydrophobic clusters at the surface of the cysteine-rich domain or by sequential hydrophobic residues in the unconstrained proline-rich domain, gives rise to the amphipathic character required for antimicrobial peptides. We hypothesize that the antimicrobial activity of penaeidins can be explained by a cooperative effect between the proline-rich and cysteine-rich features simultaneously present in their sequences.
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===Solution structure of the [T8A]-Penaeidin-3===
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Solution structure of the recombinant penaeidin-3, a shrimp antimicrobial peptide.,Yang Y, Poncet J, Garnier J, Zatylny C, Bachere E, Aumelas A J Biol Chem. 2003 Sep 19;278(38):36859-67. Epub 2003 Jul 3. PMID:12842879<ref>PMID:12842879</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_12842879}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1ueo" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 12842879 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12842879}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1UEO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Litopenaeus_vannamei Litopenaeus vannamei]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UEO OCA].
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[[Category: Penaeus vannamei]]
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[[Category: Aumelas A]]
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==Reference==
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[[Category: Bachere E]]
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Solution structure of the recombinant penaeidin-3, a shrimp antimicrobial peptide., Yang Y, Poncet J, Garnier J, Zatylny C, Bachere E, Aumelas A, J Biol Chem. 2003 Sep 19;278(38):36859-67. Epub 2003 Jul 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12842879 12842879]
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[[Category: Garnier J]]
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[[Category: Litopenaeus vannamei]]
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[[Category: Poncet J]]
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[[Category: Single protein]]
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[[Category: Yang Y]]
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[[Category: Aumelas, A.]]
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[[Category: Zatylny C]]
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[[Category: Bachere, E.]]
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[[Category: Garnier, J.]]
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[[Category: Poncet, J.]]
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[[Category: Yang, Y.]]
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[[Category: Zatylny, C.]]
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[[Category: Coil-helix-coil]]
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[[Category: Cysteine-rich]]
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[[Category: Disulfide bond]]
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[[Category: Helix]]
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[[Category: Proline-rich]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:49:54 2008''
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Current revision

Solution structure of the [T8A]-Penaeidin-3

PDB ID 1ueo

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