1utx

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:05, 9 May 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:1utx.png|left|200px]]
 
-
<!--
+
==Regulation of Cytolysin Expression by Enterococcus faecalis: Role of CylR2==
-
The line below this paragraph, containing "STRUCTURE_1utx", creates the "Structure Box" on the page.
+
<StructureSection load='1utx' size='340' side='right'caption='[[1utx]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1utx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UTX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UTX FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-
{{STRUCTURE_1utx| PDB=1utx | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1utx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1utx OCA], [https://pdbe.org/1utx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1utx RCSB], [https://www.ebi.ac.uk/pdbsum/1utx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1utx ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/Q8VL32_ENTFL Q8VL32_ENTFL]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ut/1utx_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1utx ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Enterococcus faecalis is one of the major causes for hospital-acquired antibiotic-resistant infections. It produces an exotoxin, called cytolysin, which is lethal for a wide range of Gram-positive bacteria and is toxic to higher organisms. Recently, the regulation of the cytolysin operon was connected to autoinduction by a quorum-sensing mechanism involving the CylR1/CylR2 two-component regulatory system. We report here the crystal structure of CylR2 and its properties in solution as determined by heteronuclear NMR spectroscopy. The structure reveals a rigid dimer containing a helix-turn-helix DNA-binding motif as part of a five-helix bundle that is extended by an antiparallel beta-sheet. We show that CylR2 is a DNA-binding protein that binds specifically to a 22 bp fragment of the cytolysin promoter region. NMR chemical shift perturbation experiments identify surfaces involved in DNA binding and are in agreement with a model for the CylR2/DNA complex that attributes binding specificity to a complex network of CylR2/DNA interactions. Our results propose a mechanism where repression is achieved by CylR2 obstruction of the promoter preventing biosynthesis of the cytolysin operon transcript.
-
===REGULATION OF CYTOLYSIN EXPRESSION BY ENTEROCOCCUS FAECALIS: ROLE OF CYLR2===
+
Structure and DNA-binding properties of the cytolysin regulator CylR2 from Enterococcus faecalis.,Rumpel S, Razeto A, Pillar CM, Vijayan V, Taylor A, Giller K, Gilmore MS, Becker S, Zweckstetter M EMBO J. 2004 Sep 15;23(18):3632-42. Epub 2004 Sep 9. PMID:15359276<ref>PMID:15359276</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_15359276}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 1utx" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 15359276 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_15359276}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
-
1UTX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UTX OCA].
+
-
 
+
-
==Reference==
+
-
Structure and DNA-binding properties of the cytolysin regulator CylR2 from Enterococcus faecalis., Rumpel S, Razeto A, Pillar CM, Vijayan V, Taylor A, Giller K, Gilmore MS, Becker S, Zweckstetter M, EMBO J. 2004 Sep 15;23(18):3632-42. Epub 2004 Sep 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15359276 15359276]
+
[[Category: Enterococcus faecalis]]
[[Category: Enterococcus faecalis]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Becker, S.]]
+
[[Category: Becker S]]
-
[[Category: Gilmore, M S.]]
+
[[Category: Gilmore MS]]
-
[[Category: Pillar, C M.]]
+
[[Category: Pillar CM]]
-
[[Category: Razeto, A.]]
+
[[Category: Razeto A]]
-
[[Category: Rumpel, S.]]
+
[[Category: Rumpel S]]
-
[[Category: Zweckstetter, M.]]
+
[[Category: Zweckstetter M]]
-
[[Category: Dna-binding protein]]
+
-
[[Category: Helix-turn-helix]]
+
-
[[Category: Regulation of cytolysin operon]]
+
-
[[Category: Transcriptional repressor]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:57:32 2008''
+

Current revision

Regulation of Cytolysin Expression by Enterococcus faecalis: Role of CylR2

PDB ID 1utx

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1utx"
Personal tools