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1nra

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(New page: 200px<br /><applet load="1nra" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nra" /> '''SOLUTION STRUCTURE OF AN OLD WORLD-LIKE NEUR...)
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[[Image:1nra.jpg|left|200px]]<br /><applet load="1nra" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1nra" />
 
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'''SOLUTION STRUCTURE OF AN OLD WORLD-LIKE NEUROTOXIN FROM THE VENOM OF THE NEW WORLD SCORPION CENTRUROIDES SCULPTURATUS EWING'''<br />
 
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==Overview==
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==SOLUTION STRUCTURE OF AN OLD WORLD-LIKE NEUROTOXIN FROM THE VENOM OF THE NEW WORLD SCORPION CENTRUROIDES SCULPTURATUS EWING==
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We have determined the solution structure of an alpha-toxin, CsE-V, isolated from the venom of the New World scorpion Centruroides, sculpturatus Ewing (CsE). This toxin causes spontaneous rhythmic, contractions in muscle. Unlike other New World toxins from CsE, this, protein exhibits amino acid insertions and deletions at locations similar, to Old World toxins and may thus represent a transition protein between, the New World and Old World scorpion alpha-toxins. Sequence-specific, assignments were made using 600 MHz 1H two-dimensional NMR data. NOESY, PH-COSY and amide-exchange data were used to deduce constraints for, molecular modeling calculations. Distance geometry and dynamical simulated, annealing calculations were performed to generate a family of 70, structures free of constraint violations. With respect to this family of, structures, the energy-minimized average structure had root-mean-square, deviations of 0.74 and 1.32 A for backbone and all atoms, respectively, (excluding the C-terminal dipeptide, which is disordered). As with other, scorpion toxins, the secondary structure of CsE-V consists of an, alpha-helix, a three-strand anti-parallel beta-sheet, four beta-turns, and, a hydrophobic patch that includes tyrosine residues in herringbone, configuration. Unlike the CsE-v3 and -v1 proteins from C. sculpturatus, all of the proline residues were found to be in the trans configuration., The alpha-helix is slightly longer in CsE-V. The overall structure is more, similar to the Old World alpha-toxin AaH-II from Androctonus australis, Hector (r.m.s.d 1.59 A for backbone atoms of matching residues) than to, the New World alpha-toxin CsE-v3 (r.m.s.d. 1.91 A). These structural data, on CsE-V add further to our knowledge of the conformational repertoire, exhibited by these sodium channel-binding neurotoxins.
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<StructureSection load='1nra' size='340' side='right'caption='[[1nra]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1nra]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Centruroides_sculpturatus Centruroides sculpturatus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NRA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NRA FirstGlance]. <br>
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1NRA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Centruroides_sculpturatus Centruroides sculpturatus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NRA OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nra FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nra OCA], [https://pdbe.org/1nra PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nra RCSB], [https://www.ebi.ac.uk/pdbsum/1nra PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nra ProSAT]</span></td></tr>
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==Reference==
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</table>
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Solution structure of an Old World-like neurotoxin from the venom of the New World scorpion Centruroides sculpturatus Ewing., Jablonsky MJ, Watt DD, Krishna NR, J Mol Biol. 1995 Apr 28;248(2):449-58. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7739052 7739052]
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== Function ==
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[https://www.uniprot.org/uniprot/SCXV_CENSC SCXV_CENSC] Alpha toxins bind voltage-independently at site-3 of sodium channels (Nav) and inhibit the inactivation of the activated channels, thereby blocking neuronal transmission.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nr/1nra_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nra ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Centruroides sculpturatus]]
[[Category: Centruroides sculpturatus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Jablonsky, M.J.]]
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[[Category: Jablonsky MJ]]
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[[Category: Krishna, N.R.]]
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[[Category: Krishna NR]]
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[[Category: neurotoxin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:30:14 2007''
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Current revision

SOLUTION STRUCTURE OF AN OLD WORLD-LIKE NEUROTOXIN FROM THE VENOM OF THE NEW WORLD SCORPION CENTRUROIDES SCULPTURATUS EWING

PDB ID 1nra

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