2ibp

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (10:08, 30 August 2023) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2ibp.png|left|200px]]
 
-
<!--
+
==Crystal Structure of Citrate Synthase from Pyrobaculum aerophilum==
-
The line below this paragraph, containing "STRUCTURE_2ibp", creates the "Structure Box" on the page.
+
<StructureSection load='2ibp' size='340' side='right'caption='[[2ibp]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2ibp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrobaculum_aerophilum_str._IM2 Pyrobaculum aerophilum str. IM2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IBP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IBP FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-
{{STRUCTURE_2ibp| PDB=2ibp | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ibp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ibp OCA], [https://pdbe.org/2ibp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ibp RCSB], [https://www.ebi.ac.uk/pdbsum/2ibp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ibp ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/Q8ZWP2_PYRAE Q8ZWP2_PYRAE]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ib/2ibp_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ibp ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
A growing number of organisms have been discovered inhabiting extreme environments, including temperatures in excess of 100 degrees C. How cellular proteins from such organisms retain their native folds under extreme conditions is still not fully understood. Recent computational and structural studies have identified disulfide bonding as an important mechanism for stabilizing intracellular proteins in certain thermophilic microbes. Here, we present the first proteomic analysis of intracellular disulfide bonding in the hyperthermophilic archaeon Pyrobaculum aerophilum. Our study reveals that the utilization of disulfide bonds extends beyond individual proteins to include many protein-protein complexes. We report the 1.6 A crystal structure of one such complex, a citrate synthase homodimer. The structure contains two intramolecular disulfide bonds, one per subunit, which result in the cyclization of each protein chain in such a way that the two chains are topologically interlinked, rendering them inseparable. This unusual feature emphasizes the variety and sophistication of the molecular mechanisms that can be achieved by evolution.
-
===Crystal Structure of Citrate Synthase from Pyrobaculum aerophilum===
+
Discovery of a thermophilic protein complex stabilized by topologically interlinked chains.,Boutz DR, Cascio D, Whitelegge J, Perry LJ, Yeates TO J Mol Biol. 2007 May 18;368(5):1332-44. Epub 2007 Mar 6. PMID:17395198<ref>PMID:17395198</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 2ibp" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_17395198}}, adds the Publication Abstract to the page
+
*[[Citrate Synthase 3D structures|Citrate Synthase 3D structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 17395198 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_17395198}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Large Structures]]
-
2IBP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrobaculum_aerophilum Pyrobaculum aerophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IBP OCA].
+
[[Category: Pyrobaculum aerophilum str. IM2]]
-
 
+
[[Category: Boutz DR]]
-
==Reference==
+
[[Category: Cascio D]]
-
Discovery of a thermophilic protein complex stabilized by topologically interlinked chains., Boutz DR, Cascio D, Whitelegge J, Perry LJ, Yeates TO, J Mol Biol. 2007 May 18;368(5):1332-44. Epub 2007 Mar 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17395198 17395198]
+
[[Category: Yeates TO]]
-
[[Category: Pyrobaculum aerophilum]]
+
-
[[Category: Single protein]]
+
-
[[Category: Boutz, D R.]]
+
-
[[Category: Cascio, D.]]
+
-
[[Category: Yeates, T O.]]
+
-
[[Category: Catenane]]
+
-
[[Category: Citrate synthase]]
+
-
[[Category: Disulfide bond]]
+
-
[[Category: Homodimer]]
+
-
[[Category: Thermophilic]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 20:14:31 2008''
+

Current revision

Crystal Structure of Citrate Synthase from Pyrobaculum aerophilum

PDB ID 2ibp

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ibp"
Personal tools