1nu2

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1nu2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nu2, resolution 1.90&Aring;" /> '''Crystal structure of...)
Current revision (13:28, 13 March 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1nu2.jpg|left|200px]]<br /><applet load="1nu2" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1nu2, resolution 1.90&Aring;" />
 
-
'''Crystal structure of the murine Disabled-1 (Dab1) PTB domain-ApoER2 peptide-PI-4,5P2 ternary complex'''<br />
 
-
==Overview==
+
==Crystal structure of the murine Disabled-1 (Dab1) PTB domain-ApoER2 peptide-PI-4,5P2 ternary complex==
-
Formation of the mammalian six-layered neocortex depends on a signaling, pathway that involves Reelin, the very low-density lipoprotein receptor, the apolipoprotein E receptor-2 (ApoER2), and the adaptor protein, Disabled-1 (Dab1). The 1.5 A crystal structure of a complex between the, Dab1 phosphotyrosine binding (PTB) domain and a 14-residue peptide from, the ApoER2 tail explains the unusual preference of Dab1 for, unphosphorylated tyrosine within the NPxY motif of the peptide. Crystals, of the complex soaked with the phosphoinositide PI-4,5P(2) (PI) show that, PI binds to conserved basic residues on the PTB domain opposite the, peptide binding groove. This finding explains how the Dab1 PTB domain can, simultaneously bind PI and the ApoER2 tail. Recruitment of the Dab1 PTB, domain to PI-rich regions of the plasma membrane may facilitate, association with the Reelin receptor cytoplasmic tails to transduce a, critical positional cue to migrating neurons.
+
<StructureSection load='1nu2' size='340' side='right'caption='[[1nu2]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1nu2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NU2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NU2 FirstGlance]. <br>
-
1NU2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with I3P as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NU2 OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=I3P:D-MYO-INOSITOL-1,4,5-TRIPHOSPHATE'>I3P</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nu2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nu2 OCA], [https://pdbe.org/1nu2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nu2 RCSB], [https://www.ebi.ac.uk/pdbsum/1nu2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nu2 ProSAT]</span></td></tr>
-
Origins of peptide selectivity and phosphoinositide binding revealed by structures of disabled-1 PTB domain complexes., Stolt PC, Jeon H, Song HK, Herz J, Eck MJ, Blacklow SC, Structure. 2003 May;11(5):569-79. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12737822 12737822]
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/DAB1_MOUSE DAB1_MOUSE] Adapter molecule functioning in neural development. May regulate SIAH1 activity.<ref>PMID:9009273</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nu/1nu2_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nu2 ConSurf].
 +
<div style="clear:both"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
-
[[Category: Single protein]]
+
[[Category: Blacklow SC]]
-
[[Category: Blacklow, S.C.]]
+
[[Category: Eck MJ]]
-
[[Category: Eck, M.J.]]
+
[[Category: Herz J]]
-
[[Category: Herz, J.]]
+
[[Category: Jeon H]]
-
[[Category: Jeon, H.]]
+
[[Category: Song HK]]
-
[[Category: Song, H.K.]]
+
[[Category: Stolt PC]]
-
[[Category: Stolt, P.C.]]
+
-
[[Category: I3P]]
+
-
[[Category: beta-sandwich]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:33:34 2007''
+

Current revision

Crystal structure of the murine Disabled-1 (Dab1) PTB domain-ApoER2 peptide-PI-4,5P2 ternary complex

PDB ID 1nu2

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1nu2"
Personal tools