1w36

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{{Seed}}
 
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[[Image:1w36.png|left|200px]]
 
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==RecBCD:DNA complex==
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The line below this paragraph, containing "STRUCTURE_1w36", creates the "Structure Box" on the page.
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<StructureSection load='1w36' size='340' side='right'caption='[[1w36]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1w36]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The April 2014 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''RecA and Rad51'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2014_4 10.2210/rcsb_pdb/mom_2014_4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W36 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W36 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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{{STRUCTURE_1w36| PDB=1w36 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w36 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w36 OCA], [https://pdbe.org/1w36 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w36 RCSB], [https://www.ebi.ac.uk/pdbsum/1w36 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w36 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RECB_ECOLI RECB_ECOLI] A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a rapid (>1 kb/second) and highly processive (>30 kb) ATP-dependent bidirectional helicase. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator, 5'-GCTGGTGG-3') sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to Chi site, by nicking one strand or switching the strand degraded (depending on the reaction conditions). The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang which facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination (PubMed:4562392, PubMed:4552016, PubMed:123277). In the holoenzyme this subunit contributes ATPase, 3'-5' helicase, exonuclease activity and loads RecA onto ssDNA. The RecBC complex requires the RecD subunit for nuclease activity, but can translocate along ssDNA in both directions.<ref>PMID:10197988</ref> <ref>PMID:10518611</ref> <ref>PMID:10766864</ref> <ref>PMID:123277</ref> <ref>PMID:12815437</ref> <ref>PMID:12815438</ref> <ref>PMID:1535156</ref> <ref>PMID:16041061</ref> <ref>PMID:1618858</ref> <ref>PMID:16388588</ref> <ref>PMID:18079176</ref> <ref>PMID:20852646</ref> <ref>PMID:23851395</ref> <ref>PMID:25073102</ref> <ref>PMID:4268693</ref> <ref>PMID:4552016</ref> <ref>PMID:4562392</ref> <ref>PMID:7608206</ref> <ref>PMID:9192629</ref> <ref>PMID:9230304</ref> <ref>PMID:9448271</ref> <ref>PMID:9790841</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w3/1w36_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1w36 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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RecBCD is a multi-functional enzyme complex that processes DNA ends resulting from a double-strand break. RecBCD is a bipolar helicase that splits the duplex into its component strands and digests them until encountering a recombinational hotspot (Chi site). The nuclease activity is then attenuated and RecBCD loads RecA onto the 3' tail of the DNA. Here we present the crystal structure of RecBCD bound to a DNA substrate. In this initiation complex, the DNA duplex has been split across the RecC subunit to create a fork with the separated strands each heading towards different helicase motor subunits. The strands pass along tunnels within the complex, both emerging adjacent to the nuclease domain of RecB. Passage of the 3' tail through one of these tunnels provides a mechanism for the recognition of a Chi sequence by RecC within the context of double-stranded DNA. Gating of this tunnel suggests how nuclease activity might be regulated.
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===RECBCD:DNA COMPLEX===
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Crystal structure of RecBCD enzyme reveals a machine for processing DNA breaks.,Singleton MR, Dillingham MS, Gaudier M, Kowalczykowski SC, Wigley DB Nature. 2004 Nov 11;432(7014):187-93. PMID:15538360<ref>PMID:15538360</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1w36" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_15538360}}, adds the Publication Abstract to the page
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*[[Exonuclease 3D structures|Exonuclease 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 15538360 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15538360}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1W36 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W36 OCA].
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==Reference==
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Crystal structure of RecBCD enzyme reveals a machine for processing DNA breaks., Singleton MR, Dillingham MS, Gaudier M, Kowalczykowski SC, Wigley DB, Nature. 2004 Nov 11;432(7014):187-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15538360 15538360]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Exodeoxyribonuclease V]]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Dillingham, M S.]]
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[[Category: RecA and Rad51]]
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[[Category: Gaudier, M.]]
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[[Category: C Kowalczykowski S]]
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[[Category: Kowalczykowski, S C.]]
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[[Category: Dillingham MS]]
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[[Category: Singleton, M R.]]
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[[Category: Gaudier M]]
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[[Category: Wigley, D B.]]
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[[Category: Singleton MR]]
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[[Category: Dna repair]]
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[[Category: Wigley DB]]
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[[Category: Helicase]]
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[[Category: Hydrolase]]
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[[Category: Nuclease]]
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[[Category: Recombination]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 20:49:14 2008''
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Current revision

RecBCD:DNA complex

PDB ID 1w36

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