1ny4

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(New page: 200px<br /><applet load="1ny4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ny4" /> '''Solution structure of the 30S ribosomal prot...)
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[[Image:1ny4.gif|left|200px]]<br /><applet load="1ny4" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ny4" />
 
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'''Solution structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii. Northeast Structural Genomics Consortium target JR19.'''<br />
 
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==Overview==
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==Solution structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii. Northeast Structural Genomics Consortium target JR19.==
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We report NMR assignments and solution structure of the 71-residue 30S, ribosomal protein S28E from the archaean Pyrococcus horikoshii, target, JR19 of the Northeast Structural Genomics Consortium. The structure, determined rapidly with the aid of automated backbone resonance assignment, (AutoAssign) and automated structure determination (AutoStructure), software, is characterized by a four-stranded beta-sheet with a classic, Greek-key topology and an oligonucleotide/oligosaccharide beta-barrel (OB), fold. The electrostatic surface of S28E exhibits positive and negative, patches on opposite sides, the former constituting a putative binding site, for RNA. The 13 C-terminal residues of the protein contain a consensus, sequence motif constituting the signature of the S28E protein family., Surprisingly, this C-terminal segment is unstructured in solution.
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<StructureSection load='1ny4' size='340' side='right'caption='[[1ny4]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ny4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NY4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NY4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ny4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ny4 OCA], [https://pdbe.org/1ny4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ny4 RCSB], [https://www.ebi.ac.uk/pdbsum/1ny4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ny4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RS28_PYRHO RS28_PYRHO]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ny/1ny4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ny4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We report NMR assignments and solution structure of the 71-residue 30S ribosomal protein S28E from the archaean Pyrococcus horikoshii, target JR19 of the Northeast Structural Genomics Consortium. The structure, determined rapidly with the aid of automated backbone resonance assignment (AutoAssign) and automated structure determination (AutoStructure) software, is characterized by a four-stranded beta-sheet with a classic Greek-key topology and an oligonucleotide/oligosaccharide beta-barrel (OB) fold. The electrostatic surface of S28E exhibits positive and negative patches on opposite sides, the former constituting a putative binding site for RNA. The 13 C-terminal residues of the protein contain a consensus sequence motif constituting the signature of the S28E protein family. Surprisingly, this C-terminal segment is unstructured in solution.
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==About this Structure==
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Solution NMR structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii.,Aramini JM, Huang YJ, Cort JR, Goldsmith-Fischman S, Xiao R, Shih LY, Ho CK, Liu J, Rost B, Honig B, Kennedy MA, Acton TB, Montelione GT Protein Sci. 2003 Dec;12(12):2823-30. PMID:14627742<ref>PMID:14627742</ref>
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1NY4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NY4 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Solution NMR structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii., Aramini JM, Huang YJ, Cort JR, Goldsmith-Fischman S, Xiao R, Shih LY, Ho CK, Liu J, Rost B, Honig B, Kennedy MA, Acton TB, Montelione GT, Protein Sci. 2003 Dec;12(12):2823-30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14627742 14627742]
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</div>
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[[Category: Pyrococcus horikoshii]]
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<div class="pdbe-citations 1ny4" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Acton, T.B.]]
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[[Category: Aramini, J.M.]]
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[[Category: Cort, J.R.]]
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[[Category: Ho, C.K.]]
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[[Category: Huang, Y.J.]]
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[[Category: Kennedy, M.A.]]
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[[Category: Montelione, G.T.]]
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[[Category: NESG, Northeast.Structural.Genomics.Consortium.]]
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[[Category: Shih, L.Y.]]
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[[Category: Xiao, R.]]
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[[Category: autostructure]]
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[[Category: jr19]]
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[[Category: nesg]]
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[[Category: nmr structure]]
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[[Category: northeast structural genomics consortium]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: ribosomal protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:39:07 2007''
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==See Also==
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*[[Ribosomal protein S28|Ribosomal protein S28]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Pyrococcus horikoshii]]
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[[Category: Acton TB]]
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[[Category: Aramini JM]]
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[[Category: Cort JR]]
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[[Category: Ho CK]]
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[[Category: Huang YJ]]
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[[Category: Kennedy MA]]
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[[Category: Montelione GT]]
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[[Category: Shih L-Y]]
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[[Category: Xiao R]]

Current revision

Solution structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii. Northeast Structural Genomics Consortium target JR19.

PDB ID 1ny4

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