2q20

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{{Seed}}
 
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[[Image:2q20.png|left|200px]]
 
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==Structure of the germline Vk1 O18/O8 light chain variable domain homodimer==
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The line below this paragraph, containing "STRUCTURE_2q20", creates the "Structure Box" on the page.
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<StructureSection load='2q20' size='340' side='right'caption='[[2q20]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2q20]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q20 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Q20 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2q20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q20 OCA], [https://pdbe.org/2q20 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2q20 RCSB], [https://www.ebi.ac.uk/pdbsum/2q20 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2q20 ProSAT]</span></td></tr>
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{{STRUCTURE_2q20| PDB=2q20 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/KV133_HUMAN KV133_HUMAN] V region of the variable domain of immunoglobulin light chains that participates in the antigen recognition (PubMed:24600447). Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).<ref>PMID:17576170</ref> <ref>PMID:20176268</ref> <ref>PMID:22158414</ref> <ref>PMID:24600447</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q2/2q20_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2q20 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Amyloidoses are devastating and currently incurable diseases in which the process of amyloid formation causes fatal cellular and organ damage. The molecular mechanisms underlying amyloidoses are not well known. In this study, we address the structural basis of immunoglobulin light chain amyloidosis, which results from deposition of light chains produced by clonal plasma cells. We compare light chain amyloidosis protein AL-09 to its wild-type counterpart, the kappaI O18/O8 light chain germline. Crystallographic studies indicate that both proteins form dimers. However, AL-09 has an altered dimer interface that is rotated 90 degrees from the kappaI O18/O8 dimer interface. The three non-conservative mutations in AL-09 are located within the dimer interface, consistent with their role in the decreased stability of this amyloidogenic protein. Moreover, AL-09 forms amyloid fibrils more quickly than kappaI O18/O8 in vitro. These results support the notion that the increased stability of the monomer and delayed fibril formation, together with a properly formed dimer, may be protective against amyloidogenesis. This could open a new direction into rational drug design for amyloidogenic proteins.
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===Structure of the germline Vk1 O18/O8 light chain variable domain homodimer===
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Altered dimer interface decreases stability in an amyloidogenic protein.,Baden EM, Owen BA, Peterson FC, Volkman BF, Ramirez-Alvarado M, Thompson JR J Biol Chem. 2008 Jun 6;283(23):15853-60. Epub 2008 Apr 8. PMID:18400753<ref>PMID:18400753</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_18400753}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2q20" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18400753 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18400753}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Homo sapiens]]
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q20 OCA].
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[[Category: Large Structures]]
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[[Category: Baden EM]]
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==Reference==
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[[Category: Ramirez-Alvarado M]]
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Altered dimer interface decreases stability in an amyloidogenic protein., Baden EM, Owen BA, Peterson FC, Volkman BF, Ramirez-Alvarado M, Thompson JR, J Biol Chem. 2008 Apr 8;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18400753 18400753]
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[[Category: Thompson JR]]
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[[Category: Baden, E M.]]
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[[Category: Ramirez-Alvarado, M.]]
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[[Category: Thompson, J R.]]
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[[Category: Al]]
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[[Category: Amyloid]]
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[[Category: Germline]]
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[[Category: Immunoglobulin]]
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[[Category: Light chain]]
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[[Category: Light chain amyloidosis]]
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[[Category: Light chain variable domain]]
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[[Category: Protein fibril]]
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[[Category: Vk1]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 22:38:31 2008''
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Current revision

Structure of the germline Vk1 O18/O8 light chain variable domain homodimer

PDB ID 2q20

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