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1gvy

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(New page: 200px<br /> <applet load="1gvy" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gvy, resolution 1.70&Aring;" /> '''SUBSTRATE DISTORSIO...)
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[[Image:1gvy.gif|left|200px]]<br />
 
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<applet load="1gvy" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1gvy, resolution 1.70&Aring;" />
 
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'''SUBSTRATE DISTORSION BY BETA-MANNANASE FROM PSEUDOMONAS CELLULOSA'''<br />
 
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==About this Structure==
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==Substrate distorsion by beta-mannanase from Pseudomonas cellulosa==
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1GVY is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_cellulosa Pseudomonas cellulosa]] with ZN, NA, NIN, MBF and TRS as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.78 3.2.1.78]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GVY OCA]].
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<StructureSection load='1gvy' size='340' side='right'caption='[[1gvy]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1gvy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GVY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GVY FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MBF:2-DEOXY-2-FLUORO-BETA-D-MANNOSE'>MBF</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NIN:DINITROPHENYLENE'>NIN</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gvy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gvy OCA], [https://pdbe.org/1gvy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gvy RCSB], [https://www.ebi.ac.uk/pdbsum/1gvy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gvy ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MANA_CELJU MANA_CELJU] Hydrolyzes mannan and galactomannan, but displays little activity towards other polysaccharides located in the plant cell wall. Appears to act in synergy with alpha-galactosidase (AgaA) to elicit hydrolysis of galactomannan. Preferentially hydrolyzes the larger oligosaccharides and has greater activity against non-substituted polysaccharides.<ref>PMID:7848261</ref> <ref>PMID:11064195</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gv/1gvy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gvy ConSurf].
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<div style="clear:both"></div>
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==Reference==
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==See Also==
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Substrate distortion by a beta-mannanase: snapshots of the Michaelis and covalent-intermediate complexes suggest a B(2,5) conformation for the transition state., Ducros VM, Zechel DL, Murshudov GN, Gilbert HJ, Szabo L, Stoll D, Withers SG, Davies GJ, Angew Chem Int Ed Engl. 2002 Aug 2;41(15):2824-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12203498 12203498]
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*[[Mannosidase 3D structures|Mannosidase 3D structures]]
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[[Category: Pseudomonas cellulosa]]
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== References ==
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[[Category: Single protein]]
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<references/>
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[[Category: Davies, G.J.]]
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__TOC__
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[[Category: Ducros, V.]]
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</StructureSection>
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[[Category: Gilbert, H.J.]]
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[[Category: Cellvibrio japonicus]]
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[[Category: Stoll, D.]]
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[[Category: Large Structures]]
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[[Category: Szabo, L.]]
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[[Category: Davies GJ]]
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[[Category: Withers, S.G.]]
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[[Category: Ducros V]]
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[[Category: Zechel, D.L.]]
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[[Category: Gilbert HJ]]
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[[Category: MBF]]
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[[Category: Szabo L]]
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[[Category: NA]]
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[[Category: Withers SG]]
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[[Category: NIN]]
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[[Category: Zechel DL]]
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[[Category: TRS]]
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[[Category: ZN]]
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[[Category: family 26]]
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[[Category: glycosidase]]
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[[Category: glycoside hydrolase]]
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[[Category: mannan]]
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[[Category: mannanase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 20:38:50 2007''
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Current revision

Substrate distorsion by beta-mannanase from Pseudomonas cellulosa

PDB ID 1gvy

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