1o7x

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(New page: 200px<br /><applet load="1o7x" size="450" color="white" frame="true" align="right" spinBox="true" caption="1o7x, resolution 2.7&Aring;" /> '''CITRATE SYNTHASE FROM...)
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[[Image:1o7x.jpg|left|200px]]<br /><applet load="1o7x" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1o7x, resolution 2.7&Aring;" />
 
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'''CITRATE SYNTHASE FROM SULFOLOBUS SOLFATARICUS'''<br />
 
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==Overview==
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==Citrate synthase from Sulfolobus solfataricus==
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The crystal structure of citrate synthase from the thermophilic Archaeon, Sulfolobus solfataricus (optimum growth temperature = 85 degrees C) has, been determined, extending the number of crystal structures of citrate, synthase from different organisms to a total of five that span the, temperature range over which life exists (from psychrophile to, hyperthermophile). Detailed structural analysis has revealed possible, molecular mechanisms that determine the different stabilities of the five, proteins. The key to these mechanisms is the precise structural location, of the additional interactions. As one ascends the temperature ladder, the, subunit interface of this dimeric enzyme and loop regions are reinforced, by complex electrostatic interactions, and there is a reduced exposure of, hydrophobic surface. These observations reveal a progressive pattern of, stabilization through multiple additional interactions at solvent exposed, loop and interfacial regions.
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<StructureSection load='1o7x' size='340' side='right'caption='[[1o7x]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1o7x]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O7X FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7x OCA], [https://pdbe.org/1o7x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o7x RCSB], [https://www.ebi.ac.uk/pdbsum/1o7x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o7x ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CISY_SACS2 CISY_SACS2]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o7/1o7x_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o7x ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of citrate synthase from the thermophilic Archaeon Sulfolobus solfataricus (optimum growth temperature = 85 degrees C) has been determined, extending the number of crystal structures of citrate synthase from different organisms to a total of five that span the temperature range over which life exists (from psychrophile to hyperthermophile). Detailed structural analysis has revealed possible molecular mechanisms that determine the different stabilities of the five proteins. The key to these mechanisms is the precise structural location of the additional interactions. As one ascends the temperature ladder, the subunit interface of this dimeric enzyme and loop regions are reinforced by complex electrostatic interactions, and there is a reduced exposure of hydrophobic surface. These observations reveal a progressive pattern of stabilization through multiple additional interactions at solvent exposed, loop and interfacial regions.
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==About this Structure==
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Stepwise adaptations of citrate synthase to survival at life's extremes. From psychrophile to hyperthermophile.,Bell GS, Russell RJ, Connaris H, Hough DW, Danson MJ, Taylor GL Eur J Biochem. 2002 Dec;269(24):6250-60. PMID:12473121<ref>PMID:12473121</ref>
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1O7X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Active as [http://en.wikipedia.org/wiki/Citrate_(Si)-synthase Citrate (Si)-synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.1 2.3.3.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1O7X OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Stepwise adaptations of citrate synthase to survival at life's extremes. From psychrophile to hyperthermophile., Bell GS, Russell RJ, Connaris H, Hough DW, Danson MJ, Taylor GL, Eur J Biochem. 2002 Dec;269(24):6250-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12473121 12473121]
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</div>
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[[Category: Citrate (Si)-synthase]]
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<div class="pdbe-citations 1o7x" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Sulfolobus solfataricus]]
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[[Category: Bell, G.S.]]
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[[Category: Connaris, H.]]
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[[Category: Danson, M.J.]]
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[[Category: Hough, D.W.]]
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[[Category: Russell, R.J.M.]]
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[[Category: Taylor, G.L.]]
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[[Category: lyase]]
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[[Category: tricarboxylic acid cycle]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:52:02 2007''
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==See Also==
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*[[Citrate Synthase 3D structures|Citrate Synthase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Saccharolobus solfataricus]]
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[[Category: Bell GS]]
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[[Category: Connaris H]]
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[[Category: Danson MJ]]
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[[Category: Hough DW]]
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[[Category: Russell RJM]]
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[[Category: Taylor GL]]

Current revision

Citrate synthase from Sulfolobus solfataricus

PDB ID 1o7x

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