1uam

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{{Seed}}
 
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[[Image:1uam.png|left|200px]]
 
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==Crystal structure of tRNA(m1G37)methyltransferase: Insight into tRNA recognition==
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The line below this paragraph, containing "STRUCTURE_1uam", creates the "Structure Box" on the page.
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<StructureSection load='1uam' size='340' side='right'caption='[[1uam]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1uam]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UAM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UAM FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
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{{STRUCTURE_1uam| PDB=1uam | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uam OCA], [https://pdbe.org/1uam PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uam RCSB], [https://www.ebi.ac.uk/pdbsum/1uam PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uam ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TRMD_HAEIN TRMD_HAEIN] Specifically methylates guanosine-37 in various tRNAs (By similarity).[HAMAP-Rule:MF_00605]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ua/1uam_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uam ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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tRNA(m(1)G37)methyltransferase (TrmD) catalyzes the transfer of a methyl group from S-adenosyl-L- methionine (AdoMet) to G(37) within a subset of bacterial tRNA species, which have a G residue at the 36th position. The modified guanosine is adjacent to and 3' of the anticodon and is essential for the maintenance of the correct reading frame during translation. Here we report four crystal structures of TrmD from Haemophilus influenzae, as binary complexes with either AdoMet or S-adenosyl-L-homocysteine (AdoHcy), as a ternary complex with AdoHcy and phosphate, and as an apo form. This first structure of TrmD indicates that it functions as a dimer. It also suggests the binding mode of G(36)G(37) in the active site of TrmD and the catalytic mechanism. The N-terminal domain has a trefoil knot, in which AdoMet or AdoHcy is bound in a novel, bent conformation. The C-terminal domain shows structural similarity to trp repressor. We propose a plausible model for the TrmD(2)-tRNA(2) complex, which provides insights into recognition of the general tRNA structure by TrmD.
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===Crystal structure of tRNA(m1G37)methyltransferase: Insight into tRNA recognition===
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Crystal structure of tRNA(m1G37)methyltransferase: insights into tRNA recognition.,Ahn HJ, Kim HW, Yoon HJ, Lee BI, Suh SW, Yang JK EMBO J. 2003 Jun 2;22(11):2593-603. PMID:12773376<ref>PMID:12773376</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1uam" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_12773376}}, adds the Publication Abstract to the page
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*[[TRNA methyltransferase 3D structures|TRNA methyltransferase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 12773376 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12773376}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1UAM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UAM OCA].
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==Reference==
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Crystal structure of tRNA(m1G37)methyltransferase: insights into tRNA recognition., Ahn HJ, Kim HW, Yoon HJ, Lee BI, Suh SW, Yang JK, EMBO J. 2003 Jun 2;22(11):2593-603. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12773376 12773376]
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[[Category: Haemophilus influenzae]]
[[Category: Haemophilus influenzae]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Ahn, H J.]]
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[[Category: Ahn HJ]]
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[[Category: Kim, H W.]]
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[[Category: Kim H-W]]
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[[Category: Lee, B I.]]
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[[Category: Lee BI]]
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[[Category: Suh, S W.]]
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[[Category: Suh SW]]
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[[Category: Yang, J K.]]
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[[Category: Yang JK]]
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[[Category: Yoon, H J.]]
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[[Category: Yoon H-J]]
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[[Category: Methyltransferase]]
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[[Category: Spout class]]
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[[Category: Trmd]]
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[[Category: Trna modification]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:19:35 2008''
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Current revision

Crystal structure of tRNA(m1G37)methyltransferase: Insight into tRNA recognition

PDB ID 1uam

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