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1onc

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(New page: 200px<br /><applet load="1onc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1onc, resolution 1.7&Aring;" /> '''THE REFINED 1.7 ANGST...)
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[[Image:1onc.jpg|left|200px]]<br /><applet load="1onc" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1onc, resolution 1.7&Aring;" />
 
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'''THE REFINED 1.7 ANGSTROMS X-RAY CRYSTALLOGRAPHIC STRUCTURE OF P-30, AN AMPHIBIAN RIBONUCLEASE WITH ANTI-TUMOR ACTIVITY'''<br />
 
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==Overview==
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==THE REFINED 1.7 ANGSTROMS X-RAY CRYSTALLOGRAPHIC STRUCTURE OF P-30, AN AMPHIBIAN RIBONUCLEASE WITH ANTI-TUMOR ACTIVITY==
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The X-ray crystallographic structure of P-30 protein (Onconase) has been, solved by multiple isomorphous replacement and the structure has been, refined at 1.7 A resolution to a conventional R-factor of 0.178. The, molecular model comprises all 826 non-hydrogen protein atoms, 96 solvent, molecules and a sulfate anion that is bound at the active site. The, molecular structure is similar to that of ribonuclease A. The active site, cleft is located at the junction of two three-stranded beta-sheets and the, N-terminal helix. A sulfate anion is non-covalently bound by Lys9, His10, His97, Phe98 and an intermolecular contact involving Lys55' from a, neighboring molecule. The N-terminal pyroglutamyl (Pyr) residue is part of, the active site and its O epsilon 1 atom forms a hydrogen bond with the, Lys9 N zeta. The previously constructed comparative molecular model of, P-30 based on ribonuclease A correctly predicted the overall fold of P-30, and the conformation of its active site residues. The model failed to, predict the conformation of Pyr1 and the conformation of the two loops, following helix alpha 3 and strand beta 3.
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<StructureSection load='1onc' size='340' side='right'caption='[[1onc]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1onc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lithobates_pipiens Lithobates pipiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ONC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ONC FirstGlance]. <br>
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1ONC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rana_pipiens Rana pipiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ONC OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1onc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1onc OCA], [https://pdbe.org/1onc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1onc RCSB], [https://www.ebi.ac.uk/pdbsum/1onc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1onc ProSAT]</span></td></tr>
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Refined 1.7 A X-ray crystallographic structure of P-30 protein, an amphibian ribonuclease with anti-tumor activity., Mosimann SC, Ardelt W, James MN, J Mol Biol. 1994 Mar 4;236(4):1141-53. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8120892 8120892]
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</table>
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[[Category: Rana pipiens]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/RNP30_LITPI RNP30_LITPI] Basic protein with antiproliferative/cytotoxic activity against several tumor cell lines in vitro, as well as antitumor in vivo. It exhibits a ribonuclease-like activity against high molecular weight ribosomal RNA.
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[[Category: Ardelt, W.]]
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== Evolutionary Conservation ==
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[[Category: James, M.N.G.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Mosimann, S.C.]]
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Check<jmol>
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[[Category: SO4]]
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<jmolCheckbox>
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[[Category: pancreatic ribonuclease]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/on/1onc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:02:06 2007''
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1onc ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Lithobates pipiens]]
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[[Category: Ardelt W]]
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[[Category: James MNG]]
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[[Category: Mosimann SC]]

Current revision

THE REFINED 1.7 ANGSTROMS X-RAY CRYSTALLOGRAPHIC STRUCTURE OF P-30, AN AMPHIBIAN RIBONUCLEASE WITH ANTI-TUMOR ACTIVITY

PDB ID 1onc

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