1y14

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[[Image:1y14.png|left|200px]]
 
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==Crystal structure of yeast subcomplex of Rpb4 and Rpb7==
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The line below this paragraph, containing "STRUCTURE_1y14", creates the "Structure Box" on the page.
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<StructureSection load='1y14' size='340' side='right'caption='[[1y14]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1y14]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y14 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y14 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y14 OCA], [https://pdbe.org/1y14 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y14 RCSB], [https://www.ebi.ac.uk/pdbsum/1y14 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y14 ProSAT]</span></td></tr>
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{{STRUCTURE_1y14| PDB=1y14 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RPB4_YEAST RPB4_YEAST] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA. The RPB4-RPB7 subcomplex is necessary for promoter-directed transcription initiation but is not required for recruitment of Pol II to active preinitiation complexes and seems to be dispensable for transcription elongation and termination. The RPB4-RPB7 subcomplex recruits FCP1 to Pol II. Involved in DNA repair of damage in the transcribed strand. RPB4 is dispensable under optimal growth conditions, but becomes essential during heat or cold shock and under nutrient depletion. Suppresses the RBP9-mediated transcription-coupled repair (TCR) subpathway of nucleotide excision repair (NER) but facilitates the RAD26-mediated TCR subpathway. Under stress conditions only, involved in mRNA export to the cytoplasm. Involved in mRNA decay. Promotes or enhances the deadenylation process of specific mRNAs and may recruit PAT1 and the LSM1-7 complex to these mRNAs, thus stimulating their decapping and further decay.<ref>PMID:1985924</ref> <ref>PMID:11087726</ref> <ref>PMID:11382749</ref> <ref>PMID:12411509</ref> <ref>PMID:12857861</ref> <ref>PMID:15304220</ref> <ref>PMID:16357218</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/y1/1y14_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1y14 ConSurf].
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<div style="clear:both"></div>
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===Crystal structure of yeast subcomplex of Rpb4 and Rpb7===
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==See Also==
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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== References ==
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The line below this paragraph, {{ABSTRACT_PUBMED_15591044}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 15591044 is the PubMed ID number.
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</StructureSection>
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[[Category: Large Structures]]
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{{ABSTRACT_PUBMED_15591044}}
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==About this Structure==
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1Y14 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y14 OCA].
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==Reference==
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Structures of complete RNA polymerase II and its subcomplex, Rpb4/7., Armache KJ, Mitterweger S, Meinhart A, Cramer P, J Biol Chem. 2005 Feb 25;280(8):7131-4. Epub 2004 Dec 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15591044 15591044]
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[[Category: DNA-directed RNA polymerase]]
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[[Category: Protein complex]]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Armache, K J.]]
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[[Category: Armache K-J]]
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[[Category: Cramer, P.]]
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[[Category: Cramer P]]
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[[Category: Meinhart, A.]]
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[[Category: Meinhart A]]
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[[Category: Mitterweger, S.]]
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[[Category: Mitterweger S]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:32:00 2008''
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Current revision

Crystal structure of yeast subcomplex of Rpb4 and Rpb7

PDB ID 1y14

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