1z69

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{{Seed}}
 
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[[Image:1z69.png|left|200px]]
 
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==Crystal structure of methylenetetrahydromethanopterin reductase (Mer) in complex with coenzyme F420==
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The line below this paragraph, containing "STRUCTURE_1z69", creates the "Structure Box" on the page.
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<StructureSection load='1z69' size='340' side='right'caption='[[1z69]], [[Resolution|resolution]] 2.61&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1z69]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanosarcina_barkeri Methanosarcina barkeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z69 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z69 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.61&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PG:2-(2-{2-[2-(2-METHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHANOL'>1PG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=F42:COENZYME+F420'>F42</scene></td></tr>
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{{STRUCTURE_1z69| PDB=1z69 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z69 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z69 OCA], [https://pdbe.org/1z69 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z69 RCSB], [https://www.ebi.ac.uk/pdbsum/1z69 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z69 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MER_METBF MER_METBF] Catalyzes the reversible reduction of methylene-H(4)MPT to methyl-H(4)MPT.[HAMAP-Rule:MF_01091]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z6/1z69_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1z69 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Methylenetetratetrahydromethanopterin reductase (Mer) is involved in CO(2) reduction to methane in methanogenic archaea and catalyses the reversible reduction of methylenetetrahydromethanopterin (methylene-H(4)MPT) to methyl-H(4)MPT with coenzyme F(420)H(2), which is a reduced 5'-deazaflavin. Mer was recently established as a TIM barrel structure containing a nonprolyl cis-peptide bond but the binding site of the substrates remained elusive. We report here on the crystal structure of Mer in complex with F(420) at 2.6 A resolution. The isoalloxazine ring is present in a pronounced butterfly conformation, being induced from the Re-face of F(420) by a bulge that contains the non-prolyl cis-peptide bond. The bindingmode of F(420) is very similar to that in F(420)-dependent alcohol dehydrogenase Adf despite the low sequence identity of 21%. Moreover, binding of F(420) to the apoenzyme was only associated with minor conformational changes of the polypeptide chain. These findings allowed us to build an improved model of FMN into its binding site in bacterial luciferase, which belongs to the same structural family as Mer and Adf and also contains a nonprolyl cis-peptide bond in an equivalent position.
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===Crystal structure of methylenetetrahydromethanopterin reductase (Mer) in complex with coenzyme F420===
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Crystal structure of methylenetetrahydromethanopterin reductase (Mer) in complex with coenzyme F420: Architecture of the F420/FMN binding site of enzymes within the nonprolyl cis-peptide containing bacterial luciferase family.,Aufhammer SW, Warkentin E, Ermler U, Hagemeier CH, Thauer RK, Shima S Protein Sci. 2005 Jul;14(7):1840-9. Epub 2005 Jun 3. PMID:15937276<ref>PMID:15937276</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_15937276}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1z69" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 15937276 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15937276}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1Z69 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanosarcina_barkeri Methanosarcina barkeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z69 OCA].
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==Reference==
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Crystal structure of methylenetetrahydromethanopterin reductase (Mer) in complex with coenzyme F420: Architecture of the F420/FMN binding site of enzymes within the nonprolyl cis-peptide containing bacterial luciferase family., Aufhammer SW, Warkentin E, Ermler U, Hagemeier CH, Thauer RK, Shima S, Protein Sci. 2005 Jul;14(7):1840-9. Epub 2005 Jun 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15937276 15937276]
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[[Category: 5,10-methylenetetrahydromethanopterin reductase]]
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[[Category: Methanosarcina barkeri]]
[[Category: Methanosarcina barkeri]]
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[[Category: Single protein]]
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[[Category: Aufhammer SW]]
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[[Category: Aufhammer, S W.]]
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[[Category: Ermler U]]
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[[Category: Ermler, U.]]
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[[Category: Hagemeier CH]]
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[[Category: Hagemeier, C H.]]
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[[Category: Shima S]]
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[[Category: Shima, S.]]
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[[Category: Thauer RK]]
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[[Category: Thauer, R K.]]
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[[Category: Warkentin E]]
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[[Category: Warkentin, E.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:49:33 2008''
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Current revision

Crystal structure of methylenetetrahydromethanopterin reductase (Mer) in complex with coenzyme F420

PDB ID 1z69

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