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1or7

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(New page: 200px<br /><applet load="1or7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1or7, resolution 2.00&Aring;" /> '''Crystal Structure of...)
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[[Image:1or7.gif|left|200px]]<br /><applet load="1or7" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1or7, resolution 2.00&Aring;" />
 
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'''Crystal Structure of Escherichia coli sigmaE with the Cytoplasmic Domain of its Anti-sigma RseA'''<br />
 
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==Overview==
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==Crystal Structure of Escherichia coli sigmaE with the Cytoplasmic Domain of its Anti-sigma RseA==
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The sigma factors are the key regulators of bacterial transcription. ECF, (extracytoplasmic function) sigma's are the largest and most divergent, group of sigma(70) family members. ECF sigma's are normally sequestered in, an inactive complex by their specific anti-sigma factor, which often spans, the inner membrane. Here, we determined the 2 A resolution crystal, structure of the Escherichia coli ECF sigma factor sigma(E) in an, inhibitory complex with the cytoplasmic domain of its anti-sigma, RseA., Despite extensive sequence variability, the two major domains of sigma(E), are virtually identical in structure to the corresponding domains of other, sigma(70) family members. In combination with a model of the sigma(E), holoenzyme and biochemical data, the structure reveals that RseA functions, by sterically occluding the two primary binding determinants on sigma(E), for core RNA polymerase.
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<StructureSection load='1or7' size='340' side='right'caption='[[1or7]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1or7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OR7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OR7 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1or7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1or7 OCA], [https://pdbe.org/1or7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1or7 RCSB], [https://www.ebi.ac.uk/pdbsum/1or7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1or7 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RPOE_ECOLI RPOE_ECOLI] Sigma factors are initiation factors that promote the attachment of RNA polymerase (RNAP) to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma-E controls the envelope stress response, responding to periplasmic protein stress, increased levels of periplasmic lipopolysaccharide (LPS) as well as heat shock and oxidative stress; it controls protein processing in the extracytoplasmic compartment. The 90 member regulon consists of the genes necessary for the synthesis and maintenance of both proteins and LPS of the outer membrane.<ref>PMID:7889935</ref> <ref>PMID:7889934</ref> <ref>PMID:2691330</ref> <ref>PMID:9159522</ref> <ref>PMID:9159523</ref> <ref>PMID:16336047</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/or/1or7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1or7 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1OR7 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OR7 OCA].
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*[[Sigma factor 3D structures|Sigma factor 3D structures]]
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== References ==
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==Reference==
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<references/>
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Crystal structure of Escherichia coli sigmaE with the cytoplasmic domain of its anti-sigma RseA., Campbell EA, Tupy JL, Gruber TM, Wang S, Sharp MM, Gross CA, Darst SA, Mol Cell. 2003 Apr;11(4):1067-78. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12718891 12718891]
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Campbell, E.A.]]
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[[Category: Campbell EA]]
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[[Category: Darst, S.A.]]
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[[Category: Darst SA]]
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[[Category: Gross, C.A.]]
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[[Category: Gross CA]]
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[[Category: Gruber, T.M.]]
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[[Category: Gruber TM]]
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[[Category: Sharp, M.M.]]
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[[Category: Sharp MM]]
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[[Category: Tupy, J.L.]]
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[[Category: Tupy JL]]
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[[Category: Wang, S.]]
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[[Category: Wang S]]
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[[Category: dna-binding]]
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[[Category: regulation]]
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[[Category: transmembrane]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:07:19 2007''
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Current revision

Crystal Structure of Escherichia coli sigmaE with the Cytoplasmic Domain of its Anti-sigma RseA

PDB ID 1or7

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