1y1b

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{{Seed}}
 
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[[Image:1y1b.png|left|200px]]
 
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==Solution structure of Anemonia elastase inhibitor==
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The line below this paragraph, containing "STRUCTURE_1y1b", creates the "Structure Box" on the page.
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<StructureSection load='1y1b' size='340' side='right'caption='[[1y1b]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1y1b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Anemonia_sulcata Anemonia sulcata]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y1B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y1B FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y1b OCA], [https://pdbe.org/1y1b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y1b RCSB], [https://www.ebi.ac.uk/pdbsum/1y1b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y1b ProSAT]</span></td></tr>
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{{STRUCTURE_1y1b| PDB=1y1b | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IELA_ANESU IELA_ANESU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/y1/1y1b_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1y1b ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Anemonia elastase inhibitor (AEI) is a "nonclassical" Kazal-type elastase inhibitor from Anemonia sulcata. Unlike many nonclassical inhibitors, AEI does not have a cystine-stabilized alpha-helical (CSH) motif in the sequence. We chemically synthesized AEI and determined its three-dimensional solution structure by two-dimensional NMR spectroscopy. The resulting structure of AEI was characterized by a central alpha-helix and a three-stranded antiparallel beta-sheet of a typical Kazal-type inhibitor such as silver pheasant ovomucoid third domain (OMSVP3), even though the first and fifth half-cystine residues forming a disulfide bond in AEI are shifted both toward the C-terminus in comparison with those of OMSVP3. Synthesized AEI exhibited unexpected strong inhibition toward Streptomyces griseus protease B (SGPB). Our previous study [Hemmi, H., et al. (2003) Biochemistry 42, 2524-2534] demonstrated that the site-specific introduction of the engineered disulfide bond into the OMSVP3 molecule to form the CSH motif could produce an inhibitor with a narrower specificity. Thus, the CSH motif-containing derivative of AEI (AEI analogue) was chemically synthesized when a Cys(4)-Cys(34) bond was changed to a Cys(6)-Cys(31) bond. The AEI analogue scarcely inhibited porcine pancreatic elastase (PPE), even though it exhibited almost the same potent inhibitory activity toward SGPB. For the molecular scaffold, essentially no structural difference was detected between the two, but the N-terminal loop from Pro(5) to Ile(7) near the putative reactive site (Met(10)-Gln(11)) in the analogue moved by 3.7 A toward the central helix to form the introduced Cys(6)-Cys(31) bond. Such a conformational change in the restricted region correlates with the specificity change of the inhibitor.
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===Solution structure of Anemonia elastase inhibitor===
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Structural and functional study of an Anemonia elastase inhibitor, a "nonclassical" Kazal-type inhibitor from Anemonia sulcata.,Hemmi H, Kumazaki T, Yoshizawa-Kumagaye K, Nishiuchi Y, Yoshida T, Ohkubo T, Kobayashi Y Biochemistry. 2005 Jul 19;44(28):9626-36. PMID:16008348<ref>PMID:16008348</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_16008348}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1y1b" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 16008348 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16008348}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Anemonia sulcata]]
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1Y1B is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y1B OCA].
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[[Category: Large Structures]]
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[[Category: Hemmi H]]
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==Reference==
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[[Category: Kobayashi Y]]
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Structural and functional study of an Anemonia elastase inhibitor, a "nonclassical" Kazal-type inhibitor from Anemonia sulcata., Hemmi H, Kumazaki T, Yoshizawa-Kumagaye K, Nishiuchi Y, Yoshida T, Ohkubo T, Kobayashi Y, Biochemistry. 2005 Jul 19;44(28):9626-36. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16008348 16008348]
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[[Category: Kumazaki T]]
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[[Category: Single protein]]
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[[Category: Nishiuchi Y]]
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[[Category: Hemmi, H.]]
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[[Category: Ohkubo T]]
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[[Category: Kobayashi, Y.]]
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[[Category: Yoshida T]]
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[[Category: Kumazaki, T.]]
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[[Category: Yoshizawa-Kumagaye K]]
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[[Category: Nishiuchi, Y.]]
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[[Category: Ohkubo, T.]]
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[[Category: Yoshida, T.]]
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[[Category: Yoshizawa-Kumagaye, K.]]
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[[Category: Csh motif]]
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[[Category: Kazal-type]]
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[[Category: Non-classical]]
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[[Category: Protease inhibitor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:04:47 2008''
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Current revision

Solution structure of Anemonia elastase inhibitor

PDB ID 1y1b

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