1oyg

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(New page: 200px<br /><applet load="1oyg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1oyg, resolution 1.50&Aring;" /> '''Crystal structure of...)
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[[Image:1oyg.jpg|left|200px]]<br /><applet load="1oyg" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1oyg, resolution 1.50&Aring;" />
 
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'''Crystal structure of Bacillus subtilis levansucrase'''<br />
 
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==Overview==
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==Crystal structure of Bacillus subtilis levansucrase==
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Many bacteria and about 40,000 plant species form primary carbohydrate, reserves based on fructan; these polymers of beta-D-fructofuranose are, thought to confer tolerance to drought and frost in plants. Microbial, fructan, the beta(2,6)-linked levan, is synthesized directly from sucrose, by levansucrase, which is able to catalyze both sucrose hydrolysis and, levan polymerization. The crystal structure of Bacillus subtilis, levansucrase, determined to a resolution of 1.5 A, shows a rare five-fold, beta-propeller topology with a deep, negatively charged central pocket., Arg360, a residue essential for polymerase activity, lies in a, solvent-exposed site adjacent to the central pocket. Mutagenesis data and, the sucrose-bound structure of inactive levansucrase E342A, at a, resolution of 2.1 A, strongly suggest that three conserved acidic side, chains in the central pocket are critical for catalysis, and presumably, function as nucleophile (Asp86) and general acid (Glu342), or stabilize, the transition state (Asp247).
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<StructureSection load='1oyg' size='340' side='right'caption='[[1oyg]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1oyg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OYG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OYG FirstGlance]. <br>
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1OYG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with CA and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Levansucrase Levansucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.10 2.4.1.10] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OYG OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oyg OCA], [https://pdbe.org/1oyg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oyg RCSB], [https://www.ebi.ac.uk/pdbsum/1oyg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oyg ProSAT]</span></td></tr>
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Structural framework of fructosyl transfer in Bacillus subtilis levansucrase., Meng G, Futterer K, Nat Struct Biol. 2003 Nov;10(11):935-41. Epub 2003 Sep 28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14517548 14517548]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SACB_BACSU SACB_BACSU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oy/1oyg_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oyg ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Levansucrase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Futterer K]]
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[[Category: Futterer, K.]]
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[[Category: Meng G]]
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[[Category: Meng, G.]]
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[[Category: CA]]
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[[Category: EDO]]
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[[Category: beta-propeller]]
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[[Category: glycoside hydrolase]]
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[[Category: levan]]
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[[Category: levansucrase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:17:59 2007''
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Current revision

Crystal structure of Bacillus subtilis levansucrase

PDB ID 1oyg

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