2fxp

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{{Seed}}
 
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[[Image:2fxp.png|left|200px]]
 
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==Solution Structure of the SARS-Coronavirus HR2 Domain==
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The line below this paragraph, containing "STRUCTURE_2fxp", creates the "Structure Box" on the page.
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<StructureSection load='2fxp' size='340' side='right'caption='[[2fxp]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2fxp]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome-related_coronavirus Severe acute respiratory syndrome-related coronavirus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FXP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FXP FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fxp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fxp OCA], [https://pdbe.org/2fxp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fxp RCSB], [https://www.ebi.ac.uk/pdbsum/2fxp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fxp ProSAT]</span></td></tr>
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{{STRUCTURE_2fxp| PDB=2fxp | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SPIKE_SARS SPIKE_SARS] May down-regulate host tetherin (BST2) by lysosomal degradation, thereby counteracting its antiviral activity.<ref>PMID:31199522</ref> Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 and CLEC4M/DC-SIGNR receptors and internalization of the virus into the endosomes of the host cell induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membrane fusion within endosomes.[HAMAP-Rule:MF_04099]<ref>PMID:14670965</ref> <ref>PMID:15496474</ref> Mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.[HAMAP-Rule:MF_04099] Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.[HAMAP-Rule:MF_04099]<ref>PMID:19321428</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fx/2fxp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fxp ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The envelope glycoprotein, termed the spike protein, of severe acute respiratory syndrome coronavirus (SARS-CoV) is known to mediate viral entry. Similar to other class 1 viral fusion proteins, the heptad repeat regions of SARS-CoV spike are thought to undergo conformational changes from a prefusion form to a subsequent post-fusion form that enables fusion of the viral and host membranes. Recently, the structure of a post-fusion form of SARS-CoV spike, which consists of isolated domains of heptad repeats 1 and 2 (HR1 and HR2), has been determined by x-ray crystallography. To date there is no structural information for the prefusion conformations of SARS-CoV HR1 and HR2. In this work we present the NMR structure of the HR2 domain (residues 1141-1193) from SARS-CoV (termed S2-HR2) in the presence of the co-solvent trifluoroethanol. We find that in the absence of HR1, S2-HR2 forms a coiled coil symmetric trimer with a complex molecular mass of 18 kDa. The S2-HR2 structure, which is the first example of the prefusion form of coronavirus envelope, supports the current model of viral membrane fusion and gives insight into the design of structure-based antagonists of SARS.
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===Solution Structure of the SARS-Coronavirus HR2 Domain===
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Solution structure of the severe acute respiratory syndrome-coronavirus heptad repeat 2 domain in the prefusion state.,Hakansson-McReynolds S, Jiang S, Rong L, Caffrey M J Biol Chem. 2006 Apr 28;281(17):11965-71. Epub 2006 Feb 28. PMID:16507566<ref>PMID:16507566</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2fxp" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16507566}}, adds the Publication Abstract to the page
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*[[Sandbox 3001|Sandbox 3001]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16507566 is the PubMed ID number.
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*[[Spike protein 3D structures|Spike protein 3D structures]]
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== References ==
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{{ABSTRACT_PUBMED_16507566}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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2FXP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FXP OCA].
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[[Category: Large Structures]]
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[[Category: Severe acute respiratory syndrome-related coronavirus]]
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==Reference==
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[[Category: Caffrey M]]
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Solution structure of the severe acute respiratory syndrome-coronavirus heptad repeat 2 domain in the prefusion state., Hakansson-McReynolds S, Jiang S, Rong L, Caffrey M, J Biol Chem. 2006 Apr 28;281(17):11965-71. Epub 2006 Feb 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16507566 16507566]
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[[Category: Hakansson-McReynolds S]]
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[[Category: Human sars coronavirus]]
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[[Category: Jiang S]]
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[[Category: Single protein]]
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[[Category: Caffrey, M.]]
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[[Category: Hakansson-McReynolds, S.]]
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[[Category: Jiang, S.]]
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[[Category: Coiled-coil]]
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[[Category: Prefusion intermediate]]
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[[Category: Trimer]]
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[[Category: Virus/viral protein complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:35:48 2008''
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Current revision

Solution Structure of the SARS-Coronavirus HR2 Domain

PDB ID 2fxp

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OCA

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