1p0z

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(New page: 200px<br /><applet load="1p0z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p0z, resolution 1.60&Aring;" /> '''Sensor Kinase CitA b...)
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[[Image:1p0z.gif|left|200px]]<br /><applet load="1p0z" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1p0z, resolution 1.60&Aring;" />
 
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'''Sensor Kinase CitA binding domain'''<br />
 
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==Overview==
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==Sensor Kinase CitA binding domain==
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The integral membrane sensor kinase CitA of Klebsiella pneumoniae is part, of a two-component signal transduction system that regulates the transport, and metabolism of citrate in response to its environmental concentration., Two-component systems are widely used by bacteria for such adaptive, processes, but the stereochemistry of periplasmic ligand binding and the, mechanism of signal transduction across the membrane remain poorly, understood. The crystal structure of the CitAP periplasmic sensor domain, in complex with citrate reveals a PAS fold, a versatile ligand-binding, structural motif that has not previously been observed outside the, cytoplasm or implicated in the transduction of conformational signals, across the membrane. Citrate is bound in a pocket that is shared among, many PAS domains but that shows structural variation according to the, nature of the bound ligand. In CitAP, some of the citrate contact residues, are located in the final strand of the central beta-sheet, which is, connected to the C-terminal transmembrane helix. These secondary structure, elements thus provide a potential conformational link between the, periplasmic ligand binding site and the cytoplasmic signaling domains of, the receptor.
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<StructureSection load='1p0z' size='340' side='right'caption='[[1p0z]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1p0z]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P0Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P0Z FirstGlance]. <br>
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1P0Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae] with FLC, NA, MO7 and OMO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1P0Z OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=MO7:BIS(MU4-OXO)-BIS(MU3-OXO)-OCTAKIS(MU2-OXO)-DODECAOXO-HEPTAMOLYBDENUM+(VI)'>MO7</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=OMO:MO(VI)(=O)(OH)2+CLUSTER'>OMO</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p0z OCA], [https://pdbe.org/1p0z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p0z RCSB], [https://www.ebi.ac.uk/pdbsum/1p0z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p0z ProSAT]</span></td></tr>
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The structure of the periplasmic ligand-binding domain of the sensor kinase CitA reveals the first extracellular PAS domain., Reinelt S, Hofmann E, Gerharz T, Bott M, Madden DR, J Biol Chem. 2003 Oct 3;278(40):39189-96. Epub 2003 Jul 16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12867417 12867417]
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</table>
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[[Category: Histidine kinase]]
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== Function ==
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[https://www.uniprot.org/uniprot/CITA_KLEPN CITA_KLEPN] Member of the two-component regulatory system CitA/CitB. Probably activates CitB by phosphorylation. The periplasmic domain binds H-citrate(2-), which is essential for induction of the citrate-fermentation genes.<ref>PMID:10447894</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p0/1p0z_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p0z ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Klebsiella pneumoniae]]
[[Category: Klebsiella pneumoniae]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bott, M.]]
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[[Category: Bott M]]
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[[Category: Gerharz, T.]]
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[[Category: Gerharz T]]
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[[Category: Hofmann, E.]]
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[[Category: Hofmann E]]
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[[Category: Madden, D.R.]]
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[[Category: Madden DR]]
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[[Category: Reinelt, S.]]
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[[Category: Reinelt S]]
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[[Category: FLC]]
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[[Category: MO7]]
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[[Category: NA]]
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[[Category: OMO]]
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[[Category: kinase]]
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[[Category: transferase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:21:44 2007''
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Current revision

Sensor Kinase CitA binding domain

PDB ID 1p0z

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