1p1o

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(New page: 200px<br /><applet load="1p1o" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p1o, resolution 1.6&Aring;" /> '''Crystal structure of ...)
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[[Image:1p1o.jpg|left|200px]]<br /><applet load="1p1o" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1p1o, resolution 1.6&Aring;" />
 
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'''Crystal structure of the GluR2 ligand-binding core (S1S2J) mutant L650T in complex with quisqualate'''<br />
 
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==Overview==
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==Crystal structure of the GluR2 ligand-binding core (S1S2J) mutant L650T in complex with quisqualate==
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The (S)-2-amino-3-(3-hydroxy-5-methyl-4-isoxazole) propionic acid (AMPA), receptor discriminates between agonists in terms of binding and channel, gating; AMPA is a high-affinity full agonist, whereas kainate is a, low-affinity partial agonist. Although there is extensive literature on, the functional characterization of partial agonist activity in ion, channels, structure-based mechanisms are scarce. Here we investigate the, role of Leu-650, a binding cleft residue conserved among AMPA receptors, in maintaining agonist specificity and regulating agonist binding and, channel gating by using physiological, x-ray crystallographic, and, biochemical techniques. Changing Leu-650 to Thr yields a receptor that, responds more potently and efficaciously to kainate and less potently and, efficaciously to AMPA relative to the WT receptor. Crystal structures of, the Leu-650 to Thr mutant reveal an increase in domain closure in the, kainate-bound state and a partially closed and a fully closed conformation, in the AMPA-bound form. Our results indicate that agonists can induce a, range of conformations in the GluR2 ligand-binding core and that domain, closure is directly correlated to channel activation. The partially, closed, AMPA-bound conformation of the L650T mutant likely captures the, structure of an agonist-bound, inactive state of the receptor. Together, with previously solved structures, we have determined a mechanism of, agonist binding and subsequent conformational rearrangements.
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<StructureSection load='1p1o' size='340' side='right'caption='[[1p1o]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1p1o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P1O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P1O FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=QUS:(S)-2-AMINO-3-(3,5-DIOXO-[1,2,4]OXADIAZOLIDIN-2-YL)-PROPIONIC+ACID'>QUS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p1o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p1o OCA], [https://pdbe.org/1p1o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p1o RCSB], [https://www.ebi.ac.uk/pdbsum/1p1o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p1o ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GRIA2_RAT GRIA2_RAT] Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.<ref>PMID:9351977</ref> <ref>PMID:19265014</ref> <ref>PMID:21172611</ref> <ref>PMID:12501192</ref> <ref>PMID:12015593</ref> <ref>PMID:12872125</ref> <ref>PMID:12730367</ref> <ref>PMID:16192394</ref> <ref>PMID:15591246</ref> <ref>PMID:17018279</ref> <ref>PMID:16483599</ref> <ref>PMID:19946266</ref> <ref>PMID:21317873</ref> <ref>PMID:21846932</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p1/1p1o_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p1o ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The (S)-2-amino-3-(3-hydroxy-5-methyl-4-isoxazole) propionic acid (AMPA) receptor discriminates between agonists in terms of binding and channel gating; AMPA is a high-affinity full agonist, whereas kainate is a low-affinity partial agonist. Although there is extensive literature on the functional characterization of partial agonist activity in ion channels, structure-based mechanisms are scarce. Here we investigate the role of Leu-650, a binding cleft residue conserved among AMPA receptors, in maintaining agonist specificity and regulating agonist binding and channel gating by using physiological, x-ray crystallographic, and biochemical techniques. Changing Leu-650 to Thr yields a receptor that responds more potently and efficaciously to kainate and less potently and efficaciously to AMPA relative to the WT receptor. Crystal structures of the Leu-650 to Thr mutant reveal an increase in domain closure in the kainate-bound state and a partially closed and a fully closed conformation in the AMPA-bound form. Our results indicate that agonists can induce a range of conformations in the GluR2 ligand-binding core and that domain closure is directly correlated to channel activation. The partially closed, AMPA-bound conformation of the L650T mutant likely captures the structure of an agonist-bound, inactive state of the receptor. Together with previously solved structures, we have determined a mechanism of agonist binding and subsequent conformational rearrangements.
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==About this Structure==
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Tuning activation of the AMPA-sensitive GluR2 ion channel by genetic adjustment of agonist-induced conformational changes.,Armstrong N, Mayer M, Gouaux E Proc Natl Acad Sci U S A. 2003 May 13;100(10):5736-41. Epub 2003 May 2. PMID:12730367<ref>PMID:12730367</ref>
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1P1O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with SO4 and QUS as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1P1O OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Tuning activation of the AMPA-sensitive GluR2 ion channel by genetic adjustment of agonist-induced conformational changes., Armstrong N, Mayer M, Gouaux E, Proc Natl Acad Sci U S A. 2003 May 13;100(10):5736-41. Epub 2003 May 2. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12730367 12730367]
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</div>
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[[Category: Rattus norvegicus]]
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<div class="pdbe-citations 1p1o" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Armstrong, N.]]
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[[Category: Gouaux, E.]]
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[[Category: Mayer, M.L.]]
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[[Category: QUS]]
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[[Category: SO4]]
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[[Category: ionotropic glutamate receptor]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:23:25 2007''
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==See Also==
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*[[Glutamate receptor 3D structures|Glutamate receptor 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Rattus norvegicus]]
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[[Category: Armstrong N]]
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[[Category: Gouaux E]]
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[[Category: Mayer ML]]

Current revision

Crystal structure of the GluR2 ligand-binding core (S1S2J) mutant L650T in complex with quisqualate

PDB ID 1p1o

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