1p35

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(New page: 200px<br /><applet load="1p35" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p35, resolution 2.20&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1p35.gif|left|200px]]<br /><applet load="1p35" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1p35, resolution 2.20&Aring;" />
 
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'''CRYSTAL STRUCTURE OF BACULOVIRUS P35'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF BACULOVIRUS P35==
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The aspartate-specific caspases are critical protease effectors of, programmed cell death and consequently represent important targets for, apoptotic intervention. Baculovirus P35 is a potent substrate inhibitor of, metazoan caspases, a property that accounts for its unique effectiveness, in preventing apoptosis in phylogenetically diverse organisms. Here we, report the 2.2 A resolution crystal structure of P35, the first structure, of a protein inhibitor of the death caspases. The P35 monomer possesses a, solvent-exposed loop that projects from the protein's main beta-sheet core, and positions the requisite aspartate cleavage site at the loop's apex., Distortion or destabilization of this reactive site loop by site-directed, mutagenesis converted P35 to an efficient substrate which, unlike, wild-type P35, failed to interact stably with the target caspase or block, protease activity. Thus, cleavage alone is insufficient for caspase, inhibition. These data are consistent with a new model wherein the P35, reactive site loop participates in a unique multi-step mechanism in which, the spatial orientation of the loop with respect to the P35 core, determines post-cleavage association and stoichiometric inhibition of, target caspases.
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<StructureSection load='1p35' size='340' side='right'caption='[[1p35]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1p35]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Autographa_californica_nucleopolyhedrovirus Autographa californica nucleopolyhedrovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P35 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P35 FirstGlance]. <br>
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1P35 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Autographa_californica_nucleopolyhedrovirus Autographa californica nucleopolyhedrovirus] with PO4 and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1P35 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p35 OCA], [https://pdbe.org/1p35 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p35 RCSB], [https://www.ebi.ac.uk/pdbsum/1p35 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p35 ProSAT]</span></td></tr>
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Crystal structure of baculovirus P35: role of a novel reactive site loop in apoptotic caspase inhibition., Fisher AJ, Cruz W, Zoog SJ, Schneider CL, Friesen PD, EMBO J. 1999 Apr 15;18(8):2031-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10205157 10205157]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/P35_NPVAC P35_NPVAC] Functions as an inhibitor of the host RNA interference antiviral response. Inhibits the insect host cell apoptotic response initiated by the viral infection. Blocks as well the activity of members of the caspase family of proteases. Required for late and very late gene expression.<ref>PMID:16081248</ref> <ref>PMID:1962198</ref> <ref>PMID:26018163</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p3/1p35_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p35 ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Autographa californica nucleopolyhedrovirus]]
[[Category: Autographa californica nucleopolyhedrovirus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Delacruz, W.P.]]
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[[Category: Delacruz WP]]
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[[Category: Fisher, A.J.]]
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[[Category: Fisher AJ]]
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[[Category: Friesen, P.D.]]
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[[Category: Friesen PD]]
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[[Category: Schneider, C.L.]]
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[[Category: Schneider CL]]
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[[Category: Zoog, S.J.]]
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[[Category: Zoog SJ]]
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[[Category: EDO]]
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[[Category: PO4]]
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[[Category: apoptosis]]
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[[Category: baculovirus]]
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[[Category: cell death]]
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[[Category: p35]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:25:56 2007''
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Current revision

CRYSTAL STRUCTURE OF BACULOVIRUS P35

PDB ID 1p35

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