2zgz

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (13:36, 1 November 2023) (edit) (undo)
 
(8 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2zgz.png|left|200px]]
 
-
<!--
+
==PARM with GMPPNP==
-
The line below this paragraph, containing "STRUCTURE_2zgz", creates the "Structure Box" on the page.
+
<StructureSection load='2zgz' size='340' side='right'caption='[[2zgz]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2zgz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZGZ FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-
{{STRUCTURE_2zgz| PDB=2zgz | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zgz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zgz OCA], [https://pdbe.org/2zgz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zgz RCSB], [https://www.ebi.ac.uk/pdbsum/2zgz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zgz ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/PARM_ECOLX PARM_ECOLX] Involved in the control of plasmid partition. Required for the accurate segregation of the plasmid.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zg/2zgz_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zgz ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
ParM is a prokaryotic actin homologue, which ensures even plasmid segregation before bacterial cell division. In vivo, ParM forms a labile filament bundle that is reminiscent of the more complex spindle formed by microtubules partitioning chromosomes in eukaryotic cells. However, little is known about the underlying structural mechanism of DNA segregation by ParM filaments and the accompanying dynamic instability. Our biochemical, TIRF microscopy and high-pressure SAX observations indicate that polymerization and disintegration of ParM filaments is driven by GTP rather than ATP and that ParM acts as a GTP-driven molecular switch similar to a G protein. Image analysis of electron micrographs reveals that the ParM filament is a left-handed helix, opposed to the right-handed actin polymer. Nevertheless, the intersubunit contacts are similar to those of actin. Our atomic model of the ParM-GMPPNP filament, which also fits well to X-ray fibre diffraction patterns from oriented gels, can explain why after nucleotide release, large conformational changes of the protomer lead to a breakage of intra- and interstrand interactions, and thus to the observed disintegration of the ParM filament after DNA segregation.
-
===PARM with GMPPNP===
+
Molecular structure of the ParM polymer and the mechanism leading to its nucleotide-driven dynamic instability.,Popp D, Narita A, Oda T, Fujisawa T, Matsuo H, Nitanai Y, Iwasa M, Maeda K, Onishi H, Maeda Y EMBO J. 2008 Feb 6;27(3):570-9. Epub 2008 Jan 10. PMID:18188150<ref>PMID:18188150</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 2zgz" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_18188150}}, adds the Publication Abstract to the page
+
*[[Plasmid segregation protein ParM|Plasmid segregation protein ParM]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 18188150 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_18188150}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
-
2ZGZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGZ OCA].
+
-
 
+
-
==Reference==
+
-
Molecular structure of the ParM polymer and the mechanism leading to its nucleotide-driven dynamic instability., Popp D, Narita A, Oda T, Fujisawa T, Matsuo H, Nitanai Y, Iwasa M, Maeda K, Onishi H, Maeda Y, EMBO J. 2008 Feb 6;27(3):570-9. Epub 2008 Jan 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18188150 18188150]
+
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Fujisawa, T.]]
+
[[Category: Fujisawa T]]
-
[[Category: Iwasa, M.]]
+
[[Category: Iwasa M]]
-
[[Category: Maeda, K.]]
+
[[Category: Maeda K]]
-
[[Category: Maeda, Y.]]
+
[[Category: Maeda Y]]
-
[[Category: Matsuo, H.]]
+
[[Category: Matsuo H]]
-
[[Category: Narita, A.]]
+
[[Category: Narita A]]
-
[[Category: Nitanai, Y.]]
+
[[Category: Nitanai Y]]
-
[[Category: Oda, T.]]
+
[[Category: Oda T]]
-
[[Category: Onishi, H.]]
+
[[Category: Onishi H]]
-
[[Category: Popp, D.]]
+
[[Category: Popp D]]
-
[[Category: Parm]]
+
-
[[Category: Plasmid]]
+
-
[[Category: Plasmid partition]]
+
-
[[Category: Structural protein]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:17:13 2008''
+

Current revision

PARM with GMPPNP

PDB ID 2zgz

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2zgz"
Personal tools