2ojz

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{{Seed}}
 
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[[Image:2ojz.png|left|200px]]
 
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==Anti-DNA antibody ED10==
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The line below this paragraph, containing "STRUCTURE_2ojz", creates the "Structure Box" on the page.
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<StructureSection load='2ojz' size='340' side='right'caption='[[2ojz]], [[Resolution|resolution]] 2.73&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2ojz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OJZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OJZ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.73&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ojz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ojz OCA], [https://pdbe.org/2ojz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ojz RCSB], [https://www.ebi.ac.uk/pdbsum/2ojz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ojz ProSAT]</span></td></tr>
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{{STRUCTURE_2ojz| PDB=2ojz | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A2NHM3_MOUSE A2NHM3_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oj/2ojz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ojz ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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DNA recognition by antibodies is a key feature of autoimmune diseases, yet model systems with structural information are very limited. The monoclonal antibody ED-10 recognizes one of the strands of the DNA duplex used in the immunogenic complex. Modifications of the 5' end decrease the binding affinity and short oligonucleotides retain high binding affinity. We determined crystal structures for the Fab bound to a 6-mer oligonucleotide containing the specific sequence that raised the antibody and compared it with the unliganded Fab. Only the first two bases from the 5' end (dTdC) display electron density and we observe four key hydrogen bonds at the interface. The thymine ring is stacked between TrpH50 and TrpH95, and the cytosine ring is packed against TyrL32. Upon DNA binding, TyrH97 and TrpH95 rearrange to allow subnanomolar binding affinity, five orders of magnitude higher than other reported complexes, possibly because of having gone through affinity maturation. This structure represents the first bona fide antibody DNA immunogen complex described in atomic detail.
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===Anti-DNA antibody ED10===
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Specific recognition of a DNA immunogen by its elicited antibody.,Sanguineti S, Centeno Crowley JM, Lodeiro Merlo MF, Cerutti ML, Wilson IA, Goldbaum FA, Stanfield RL, de Prat-Gay G J Mol Biol. 2007 Jun 29;370(1):183-95. Epub 2007 Apr 24. PMID:17512945<ref>PMID:17512945</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2ojz" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_17512945}}, adds the Publication Abstract to the page
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*[[Antibody 3D structures|Antibody 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 17512945 is the PubMed ID number.
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*[[Sandbox 20009|Sandbox 20009]]
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*[[3D structures of non-human antibody|3D structures of non-human antibody]]
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{{ABSTRACT_PUBMED_17512945}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OJZ OCA].
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</StructureSection>
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[[Category: Large Structures]]
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==Reference==
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[[Category: Mus musculus]]
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Specific recognition of a DNA immunogen by its elicited antibody., Sanguineti S, Centeno Crowley JM, Lodeiro Merlo MF, Cerutti ML, Wilson IA, Goldbaum FA, Stanfield RL, de Prat-Gay G, J Mol Biol. 2007 Jun 29;370(1):183-95. Epub 2007 Apr 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17512945 17512945]
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[[Category: Sanguineti S]]
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[[Category: Prat-Gay, G de.]]
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[[Category: Stanfield RL]]
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[[Category: Sanguineti, S.]]
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[[Category: Wilson IA]]
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[[Category: Stanfield, R L.]]
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[[Category: De Prat-Gay G]]
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[[Category: Wilson, I A.]]
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[[Category: Immunoglobulin fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:21:28 2008''
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Current revision

Anti-DNA antibody ED10

PDB ID 2ojz

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