We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

1p7o

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1p7o" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p7o, resolution 2.30&Aring;" /> '''Crystal structure of...)
Current revision (06:10, 3 April 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1p7o.jpg|left|200px]]<br /><applet load="1p7o" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1p7o, resolution 2.30&Aring;" />
 
-
'''Crystal structure of phospholipase A2 (MIPLA4) from Micropechis ikaheka'''<br />
 
-
==Overview==
+
==Crystal structure of phospholipase A2 (MIPLA4) from Micropechis ikaheka==
-
Comparison of the crystal structures of three Micropechis ikaheka, phospholipase A2 isoenzymes (MiPLA2, MiPLA3 and MiPLA4, which exhibit, different levels of pharmacological effects) shows that their C-terminus, (residues 110-124) is the most variable. M-Type receptor binding affinity, of the isoenzymes has also been investigated and MiPLA4 binds to the, rabbit M-type receptor with high affinity. Examination of surface charges, of the isoenzymes reveals a trend of increase in positive charges with, potency. The isoenzymes are shown to oligomerize in a, concentration-dependent manner in a semi-denaturing gel. The C-termini of, the medium (MiPLA4) and highly potent (MiPLA2) isoenzyme molecules cluster, together, forming a highly exposed area. A BLAST search using the sequence, of the most potent MiPLA2 results in high similarity to Staphylococcus, aureus clotting factor A and cadherin 11. This might explain the, myotoxicity, anticoagulant and hemoglobinuria effects of MiPLA2s.
+
<StructureSection load='1p7o' size='340' side='right'caption='[[1p7o]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1p7o]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Micropechis_ikaheca Micropechis ikaheca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P7O FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p7o OCA], [https://pdbe.org/1p7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p7o RCSB], [https://www.ebi.ac.uk/pdbsum/1p7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p7o ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/PA2A2_TROCA PA2A2_TROCA] PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p7/1p7o_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p7o ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1P7O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Micropechis_ikaheka Micropechis ikaheka]. Active as [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1P7O OCA].
+
*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Structure and function comparison of Micropechis ikaheka snake venom phospholipase A2 isoenzymes., Lok SM, Gao R, Rouault M, Lambeau G, Gopalakrishnakone P, Swaminathan K, FEBS J. 2005 Mar;272(5):1211-20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15720395 15720395]
+
[[Category: Large Structures]]
-
[[Category: Micropechis ikaheka]]
+
[[Category: Micropechis ikaheca]]
-
[[Category: Phospholipase A(2)]]
+
[[Category: Lok SM]]
-
[[Category: Protein complex]]
+
[[Category: Swaminathan K]]
-
[[Category: Lok, S.M.]]
+
-
[[Category: Swaminathan, K.]]
+
-
[[Category: micropechis ikaheka]]
+
-
[[Category: pancreatic loop]]
+
-
[[Category: phospholipase a2]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:33:30 2007''
+

Current revision

Crystal structure of phospholipase A2 (MIPLA4) from Micropechis ikaheka

PDB ID 1p7o

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1p7o"
Personal tools