2iw0

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:40, 9 October 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2iw0.png|left|200px]]
 
-
<!--
+
==Structure of the chitin deacetylase from the fungal pathogen Colletotrichum lindemuthianum==
-
The line below this paragraph, containing "STRUCTURE_2iw0", creates the "Structure Box" on the page.
+
<StructureSection load='2iw0' size='340' side='right'caption='[[2iw0]], [[Resolution|resolution]] 1.81&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2iw0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Colletotrichum_lindemuthianum Colletotrichum lindemuthianum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IW0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IW0 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.81&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-
{{STRUCTURE_2iw0| PDB=2iw0 | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iw0 OCA], [https://pdbe.org/2iw0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iw0 RCSB], [https://www.ebi.ac.uk/pdbsum/2iw0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iw0 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/CDA_COLLN CDA_COLLN] Hydrolyzes the N-acetamido groups of N-acetyl-D-glucosamine polymers in chitin to form chitosan and acetate (PubMed:15555935, PubMed:16232493, PubMed:16878976, PubMed:7592838, PubMed:8987657, PubMed:9373940). May play a role in evasion of the host immune response; plant chitinases liberate chitin molecules from the fungal cell wall which act as elicitors of the plant immune response, deacetylation of the liberated chitin neutralizes elicitor activity (PubMed:10913295, PubMed:8987657).<ref>PMID:15555935</ref> <ref>PMID:16232493</ref> <ref>PMID:16878976</ref> <ref>PMID:7592838</ref> <ref>PMID:8987657</ref> <ref>PMID:9373940</ref> <ref>PMID:10913295</ref> <ref>PMID:8987657</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iw/2iw0_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2iw0 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The fungal pathogen Colletotrichum lindemuthianum secretes an endo-chitin de-N-acetylase (ClCDA) to modify exposed hyphal chitin during penetration and infection of plants. Although a significant amount of biochemical data is available on fungal chitin de-N-acetylases, no structural data exist. Here we describe the 1.8 A crystal structure of a ClCDA product complex and the analysis of the reaction mechanism using Hammett linear free energy relationships, subsite probing, and atomic absorption spectroscopy studies. The structural data in combination with biochemical data reveal that ClCDA consists of a single domain encompassing a mononuclear metalloenzyme which employs a conserved His-His-Asp zinc-binding triad closely associated with the conserved catalytic base (aspartic acid) and acid (histidine) to carry out acid/base catalysis. The data presented here indicate that ClCDA possesses a highly conserved substrate-binding groove, with subtle alterations that influence substrate specificity and subsite affinity. Strikingly, the structure also shows that the hexahistidine purification tag appears to form a tight interaction with the active site groove. The enzyme requires occupancy of at least the 0 and +1 subsites by (GlcNAc)(2) for activity and proceeds through a tetrahedral oxyanion intermediate.
-
===STRUCTURE OF THE CHITIN DEACETYLASE FROM THE FUNGAL PATHOGEN COLLETOTRICHUM LINDEMUTHIANUM===
+
Structure and mechanism of chitin deacetylase from the fungal pathogen Colletotrichum lindemuthianum.,Blair DE, Hekmat O, Schuttelkopf AW, Shrestha B, Tokuyasu K, Withers SG, van Aalten DM Biochemistry. 2006 Aug 8;45(31):9416-26. PMID:16878976<ref>PMID:16878976</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_16878976}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 2iw0" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 16878976 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_16878976}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Colletotrichum lindemuthianum]]
-
2IW0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Glomerella_lindemuthiana Glomerella lindemuthiana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IW0 OCA].
+
[[Category: Large Structures]]
-
 
+
[[Category: Blair DE]]
-
==Reference==
+
[[Category: Hekmat O]]
-
Structure and mechanism of chitin deacetylase from the fungal pathogen Colletotrichum lindemuthianum., Blair DE, Hekmat O, Schuttelkopf AW, Shrestha B, Tokuyasu K, Withers SG, van Aalten DM, Biochemistry. 2006 Aug 8;45(31):9416-26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16878976 16878976]
+
[[Category: Schuttelkopf AW]]
-
[[Category: Chitin deacetylase]]
+
[[Category: Shrestha B]]
-
[[Category: Glomerella lindemuthiana]]
+
[[Category: Tokuyasu K]]
-
[[Category: Single protein]]
+
[[Category: Withers SG]]
-
[[Category: Aalten, D M.F Van.]]
+
[[Category: Van Aalten DMF]]
-
[[Category: Blair, D E.]]
+
-
[[Category: Hekmat, O.]]
+
-
[[Category: Schuttelkopf, A W.]]
+
-
[[Category: Shrestha, B.]]
+
-
[[Category: Tokuyasu, K.]]
+
-
[[Category: Withers, S G.]]
+
-
[[Category: Chitin de-n-acetylase]]
+
-
[[Category: Family 4 carbohydrate esterase]]
+
-
[[Category: Hydrolase]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:33:02 2008''
+

Current revision

Structure of the chitin deacetylase from the fungal pathogen Colletotrichum lindemuthianum

PDB ID 2iw0

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2iw0"
Personal tools