1p9n

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(New page: 200px<br /><applet load="1p9n" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p9n, resolution 2.80&Aring;" /> '''Crystal structure of...)
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[[Image:1p9n.jpg|left|200px]]<br /><applet load="1p9n" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1p9n, resolution 2.80&Aring;" />
 
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'''Crystal structure of Escherichia coli MobB.'''<br />
 
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==Overview==
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==Crystal structure of Escherichia coli MobB.==
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The crystal structure of Escherichia coli MobB, an enzyme involved in the, final step of molybdenum-cofactor biosynthesis, forms intertwined dimers., Each molecule consists of two segments and requires the second monomer for, stable folding. Dimerization buries a quarter of the solvent-accessible, area of the monomer. These dimers assemble into a hexagonal lattice with, P6(4)22 symmetry and occupy only approximately 25% of the unit-cell, volume. The symmetry-related dimers associate tightly into a helical, structure with a diameter of 250 A and a pitch of 98 A. Two such helices, are intertwined, shifted by 49 A along the sixfold axis. Within the, crystal, these helices form thin-walled cylinders with an external, diameter of 250 A and an internal diameter of 190 A. Their center is, filled with solvent. These cylinders pack closely together, forming a, hexagonal lattice with the highest possible packing density. This, arrangement of dimers allows extensive intermolecular contacts with 75%, solvent content in the crystal.
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<StructureSection load='1p9n' size='340' side='right'caption='[[1p9n]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1p9n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P9N FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p9n OCA], [https://pdbe.org/1p9n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p9n RCSB], [https://www.ebi.ac.uk/pdbsum/1p9n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p9n ProSAT], [https://www.topsan.org/Proteins/BSGI/1p9n TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MOBB_ECOLI MOBB_ECOLI] GTP-binding protein that is not required for the biosynthesis of Mo-molybdopterin guanine dinucleotide (Mo-MGD) cofactor, and not necessary for the formation of active molybdoenzymes using this form of molybdenum cofactor. May act as an adapter protein to achieve the efficient biosynthesis and utilization of MGD. Displays a weak intrinsic GTPase activity. Is also able to bind the nucleotides ATP, TTP and GDP, but with lower affinity than GTP.<ref>PMID:9219527</ref> <ref>PMID:10978348</ref> <ref>PMID:12682065</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p9/1p9n_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p9n ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of Escherichia coli MobB, an enzyme involved in the final step of molybdenum-cofactor biosynthesis, forms intertwined dimers. Each molecule consists of two segments and requires the second monomer for stable folding. Dimerization buries a quarter of the solvent-accessible area of the monomer. These dimers assemble into a hexagonal lattice with P6(4)22 symmetry and occupy only approximately 25% of the unit-cell volume. The symmetry-related dimers associate tightly into a helical structure with a diameter of 250 A and a pitch of 98 A. Two such helices are intertwined, shifted by 49 A along the sixfold axis. Within the crystal, these helices form thin-walled cylinders with an external diameter of 250 A and an internal diameter of 190 A. Their center is filled with solvent. These cylinders pack closely together, forming a hexagonal lattice with the highest possible packing density. This arrangement of dimers allows extensive intermolecular contacts with 75% solvent content in the crystal.
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==About this Structure==
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Molecules of Escherichia coli MobB assemble into densely packed hollow cylinders in a crystal lattice with 75% solvent content.,Rangarajan SE, Tocilj A, Li Y, Iannuzzi P, Matte A, Cygler M Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2348-52. Epub 2003, Nov 27. PMID:14646116<ref>PMID:14646116</ref>
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1P9N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1P9N OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Molecules of Escherichia coli MobB assemble into densely packed hollow cylinders in a crystal lattice with 75% solvent content., Rangarajan SE, Tocilj A, Li Y, Iannuzzi P, Matte A, Cygler M, Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2348-52. Epub 2003, Nov 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14646116 14646116]
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</div>
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<div class="pdbe-citations 1p9n" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: BSGI, Montreal-Kingston.Bacterial.Structural.Genomics.Initiative.]]
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[[Category: Cygler M]]
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[[Category: Cygler, M.]]
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[[Category: Iannuzzi P]]
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[[Category: Iannuzzi, P.]]
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[[Category: Li Y]]
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[[Category: Li, Y.]]
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[[Category: Matte A]]
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[[Category: Matte, A.]]
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[[Category: Rangarajan SE]]
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[[Category: Rangarajan, S.E.]]
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[[Category: Tocilj A]]
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[[Category: Tocilj, A.]]
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[[Category: SO4]]
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[[Category: bsgi]]
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[[Category: crystal structure]]
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[[Category: mobb]]
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[[Category: molybdopterin cofactor biosynthesis]]
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[[Category: montreal-kingston bacterial structural genomics initiative]]
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[[Category: structural genomics]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:36:56 2007''
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Current revision

Crystal structure of Escherichia coli MobB.

PDB ID 1p9n

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