2pcf

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{{Seed}}
 
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[[Image:2pcf.png|left|200px]]
 
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==THE COMPLEX OF CYTOCHROME F AND PLASTOCYANIN DETERMINED WITH PARAMAGNETIC NMR. BASED ON THE STRUCTURES OF CYTOCHROME F AND PLASTOCYANIN, 10 STRUCTURES==
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The line below this paragraph, containing "STRUCTURE_2pcf", creates the "Structure Box" on the page.
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<StructureSection load='2pcf' size='340' side='right'caption='[[2pcf]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2pcf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Brassica_rapa Brassica rapa] and [https://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PCF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PCF FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 10 models</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr>
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{{STRUCTURE_2pcf| PDB=2pcf | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pcf OCA], [https://pdbe.org/2pcf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pcf RCSB], [https://www.ebi.ac.uk/pdbsum/2pcf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pcf ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CYF_BRARR CYF_BRARR] Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pc/2pcf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pcf ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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BACKGROUND: The reduction of plastocyanin by cytochrome f is part of the chain of photosynthetic electron transfer reactions that links photosystems II and I. The reaction is rapid and is influenced by charged residues on both proteins. Previously determined structures show that the plastocyanin copper and cytochrome f haem redox centres are some distance apart from the relevant charged sidechains, and until now it was unclear how a transient electrostatic complex can be formed that brings the redox centres sufficiently close for a rapid reaction. RESULTS: A new approach was used to determine the structure of the transient complex between cytochrome f and plastocyanin. Diamagnetic chemical shift changes and intermolecular pseudocontact shifts in the NMR spectrum of plastocyanin were used as input in restrained rigid-body molecular dynamics calculations. An ensemble of ten structures was obtained, in which the root mean square deviation of the plastocyanin position relative to cytochrome f is 1.0 A. Electrostatic interaction is maintained at the same time as the hydrophobic side of plastocyanin makes close contact with the haem area, thus providing a short electron transfer pathway (Fe-Cu distance 10.9 A) via residues Tyr1 or Phe4 (cytochrome f) and the copper ligand His87 (plastocyanin). CONCLUSIONS: The combined use of diamagnetic and paramagnetic chemical shift changes makes it possible to obtain detailed information about the structure of a transient complex of redox proteins. The structure suggests that the electrostatic interactions 'guide' the partners into a position that is optimal for electron transfer, and which may be stabilised by short-range interactions.
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===THE COMPLEX OF CYTOCHROME F AND PLASTOCYANIN DETERMINED WITH PARAMAGNETIC NMR. BASED ON THE STRUCTURES OF CYTOCHROME F AND PLASTOCYANIN, 10 STRUCTURES===
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The structure of the complex of plastocyanin and cytochrome f, determined by paramagnetic NMR and restrained rigid-body molecular dynamics.,Ubbink M, Ejdeback M, Karlsson BG, Bendall DS Structure. 1998 Mar 15;6(3):323-35. PMID:9551554<ref>PMID:9551554</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2pcf" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_9551554}}, adds the Publication Abstract to the page
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*[[Cytochrome f 3D structures|Cytochrome f 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 9551554 is the PubMed ID number.
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*[[Plastocyanin 3D structures|Plastocyanin 3D structures]]
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== References ==
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{{ABSTRACT_PUBMED_9551554}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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2PCF is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Brassica_rapa Brassica rapa] and [http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PCF OCA].
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==Reference==
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The structure of the complex of plastocyanin and cytochrome f, determined by paramagnetic NMR and restrained rigid-body molecular dynamics., Ubbink M, Ejdeback M, Karlsson BG, Bendall DS, Structure. 1998 Mar 15;6(3):323-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9551554 9551554]
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[[Category: Brassica rapa]]
[[Category: Brassica rapa]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
[[Category: Spinacia oleracea]]
[[Category: Spinacia oleracea]]
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[[Category: Bendall, D S.]]
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[[Category: Bendall DS]]
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[[Category: Ejdeback, M.]]
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[[Category: Ejdeback M]]
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[[Category: Karlsson, B G.]]
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[[Category: Karlsson BG]]
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[[Category: Ubbink, M.]]
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[[Category: Ubbink M]]
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[[Category: Chemical shift]]
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[[Category: Complex formation]]
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[[Category: Dynamic complex]]
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[[Category: Electron transport]]
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[[Category: Paramagnetic]]
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[[Category: Photosynthesis]]
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[[Category: Pseudocontact shift]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:02:56 2008''
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Current revision

THE COMPLEX OF CYTOCHROME F AND PLASTOCYANIN DETERMINED WITH PARAMAGNETIC NMR. BASED ON THE STRUCTURES OF CYTOCHROME F AND PLASTOCYANIN, 10 STRUCTURES

PDB ID 2pcf

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