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(New page: 200px<br /><applet load="1pdf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pdf" /> '''Fitting of gp11 crystal structure into 3D cr...)
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'''Fitting of gp11 crystal structure into 3D cryo-EM reconstruction of bacteriophage T4 baseplate-tail tube complex'''<br />
 
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==Overview==
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==Fitting of gp11 crystal structure into 3D cryo-EM reconstruction of bacteriophage T4 baseplate-tail tube complex==
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The baseplate of bacteriophage T4 is a multiprotein molecular machine that, controls host cell recognition, attachment, tail sheath contraction and, viral DNA ejection. We report here the three-dimensional structure of the, baseplate-tail tube complex determined to a resolution of 12 A by, cryoelectron microscopy. The baseplate has a six-fold symmetric, dome-like, structure approximately 520 A in diameter and approximately 270 A long, assembled around a central hub. A 940 A-long and 96 A-diameter tail tube, coaxial with the hub, is connected to the top of the baseplate. At the, center of the dome is a needle-like structure that was previously, identified as a cell puncturing device. We have identified the locations, of six proteins with known atomic structures, and established the position, and shape of several other baseplate proteins. The baseplate structure, suggests a mechanism of baseplate triggering and structural transition, during the initial stages of T4 infection.
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<SX load='1pdf' size='340' side='right' viewer='molstar' caption='[[1pdf]], [[Resolution|resolution]] 12.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1pdf]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PDF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PDF FirstGlance]. <br>
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1PDF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PDF OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 12&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pdf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pdf OCA], [https://pdbe.org/1pdf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pdf RCSB], [https://www.ebi.ac.uk/pdbsum/1pdf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pdf ProSAT]</span></td></tr>
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==Reference==
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</table>
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Three-dimensional structure of bacteriophage T4 baseplate., Kostyuchenko VA, Leiman PG, Chipman PR, Kanamaru S, van Raaij MJ, Arisaka F, Mesyanzhinov VV, Rossmann MG, Nat Struct Biol. 2003 Sep;10(9):688-93. Epub 2003 Aug 17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12923574 12923574]
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== Function ==
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[[Category: Bacteriophage t4]]
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[https://www.uniprot.org/uniprot/BP11_BPT4 BP11_BPT4] Baseplate protein that is part of the baseplate wedge and that connects the short tail fibers to the baseplate (PubMed:15315755). Involved in the tail assembly.<ref>PMID:15315755</ref> <ref>PMID:21129200</ref>
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Arisaka, F.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Chipman, P.R.]]
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Check<jmol>
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[[Category: Kanamaru, S.]]
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<jmolCheckbox>
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[[Category: Kostyuchenko, V.A.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pd/1pdf_consurf.spt"</scriptWhenChecked>
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[[Category: Leiman, P.G.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: Mesyanzhinov, V.V.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: Raaij, M.J.van.]]
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</jmolCheckbox>
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[[Category: Rossmann, M.G.]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pdf ConSurf].
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[[Category: structural protein]]
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<div style="clear:both"></div>
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== References ==
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:42:32 2007''
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<references/>
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__TOC__
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</SX>
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[[Category: Escherichia virus T4]]
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[[Category: Large Structures]]
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[[Category: Arisaka F]]
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[[Category: Chipman PR]]
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[[Category: Kanamaru S]]
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[[Category: Kostyuchenko VA]]
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[[Category: Leiman PG]]
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[[Category: Mesyanzhinov VV]]
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[[Category: Rossmann MG]]
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[[Category: Van Raaij MJ]]

Current revision

Fitting of gp11 crystal structure into 3D cryo-EM reconstruction of bacteriophage T4 baseplate-tail tube complex

1pdf, resolution 12.00Å

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